| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, pkn2, prk2, prkcl2 |
| Entrez GeneID | 567134 |
| WB Predicted band size | 110.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) pkn2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids DANRE pkn2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于斑马鱼(DANRE)PKN2(N-term)抗体的3篇文献示例(注：文献为虚拟示例，实际引用需核实)：
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1. **Title**: *Functional analysis of PKN2 in zebrafish embryonic development*
**Authors**: Lee S, et al.
**Summary**: 研究通过N端特异性抗体揭示了PKN2在斑马鱼原肠胚形成中的表达模式，证实其通过调控细胞极性影响胚胎形态发生。
2. **Title**: *PKN2-mediated signaling in zebrafish neural crest migration*
**Authors**: Gomez-Ramos M, et al.
**Summary**: 利用PKN2(N-term)抗体进行Western blot和免疫荧光实验，证明PKN2通过Rho/ROCK通路调控神经嵴细胞的迁移与分化。
3. **Title**: *Characterization of PKN2 knockout zebrafish using antibody-based validation*
**Authors**: Chen X, et al.
**Summary**: 开发针对PKN2 N端的多克隆抗体，验证基因敲除模型，发现PKN2缺失导致心血管发育异常。
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(实际文献需通过PubMed或Web of Science以关键词“zebrafish PKN2 antibody”或“Danio rerio PKN2 N-terminal”检索确认。)
The DANRE pkn2 (N-term) antibody is designed to target the N-terminal region of Protein Kinase N2 (PKN2) in the zebrafish (*Danio rerio*) model organism. PKN2. a member of the protein kinase C (PKC) superfamily, is a serine/threonine kinase involved in regulating diverse cellular processes, including cytoskeletal organization, cell adhesion, and signal transduction pathways. It interacts with Rho GTPases and plays roles in developmental processes, tissue morphogenesis, and cell polarity. The N-terminal region of PKN2 contains a conserved PB1 domain, which mediates protein-protein interactions critical for its function.
This antibody is typically generated using synthetic peptides or recombinant proteins corresponding to the N-terminal epitopes of zebrafish PKN2. ensuring species-specific recognition. It is widely used in immunohistochemistry, Western blotting, and immunofluorescence to study PKN2 expression, localization, and dynamics during zebrafish embryogenesis or in disease models. Researchers employ this tool to investigate PKN2's role in developmental defects, neural crest cell migration, or cardiovascular development. Validation often includes knockout controls to confirm specificity. Its application advances understanding of PKN2 signaling in vertebrate development and potential implications in human pathologies linked to kinase dysregulation.
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