| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 咨询技术 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Carbohydrate sulfotransferase 1, Keratan sulfate Gal-6 sulfotransferase, KS6ST, KSGal6ST, KSST, chst1 |
| Entrez GeneID | 445124 |
| WB Predicted band size | 48.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) chst1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 341-375 amino acids of DANRE chst1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于斑马鱼(Danio rerio)CHST1抗体的**模拟参考文献示例**(仅供参考，非真实文献)：
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1. **文献名称**: *Carbohydrate sulfotransferase 1 (chst1) is essential for zebrafish cartilage morphogenesis*
**作者**: Smith A, et al.
**摘要**: 通过抗体验证CHST1在斑马鱼胚胎软骨形成中的表达，发现其缺失导致脊索畸形和硫酸软骨素合成异常，表明CHST1在骨骼发育中起关键作用。
2. **文献名称**: *Localization and functional analysis of CHST1 in Danio rerio neural development*
**作者**: Tanaka K, et al.
**摘要**: 使用特异性抗体进行免疫组化分析，揭示CHST1在斑马鱼早期神经系统(如视顶盖)高表达，敲除后出现轴突导向缺陷，提示其参与神经细胞迁移。
3. **文献名称**: *Antibody-based profiling of sulfotransferase expression in zebrafish larval stages*
**作者**: Lee S, et al.
**摘要**: 开发并验证抗斑马鱼CHST1的多克隆抗体，通过Western blot和共聚焦显微镜证实其在幼体鳃弓及鳍褶中的动态表达模式，关联其与组织硫化的关系。
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**注意**：以上为模拟内容，如需真实文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词：
`Danio rerio CHST1 antibody`、`zebrafish chst1 sulfotransferase`，并筛选涉及抗体应用的实验研究(如免疫染色、蛋白检测等)。
The DANRE (Danio rerio) Chst1 antibody is a specialized tool used to study the zebrafish homolog of carbohydrate sulfotransferase 1 (Chst1), an enzyme critical for sulfation modifications in glycosaminoglycan (GAG) biosynthesis. Chst1 catalyzes the transfer of sulfate groups to specific positions on chondroitin sulfate (CS) and dermatan sulfate (DS), polysaccharides essential for extracellular matrix (ECM) organization, cell signaling, and tissue development. In zebrafish, Chst1 plays a vital role in embryonic development, particularly in skeletal formation, neural crest cell migration, and organogenesis. Dysregulation of Chst1 activity has been linked to developmental defects and pathological conditions, including skeletal dysplasia and impaired tissue repair.
The DANRE Chst1 antibody is typically generated using immunogenic peptides or recombinant proteins derived from zebrafish Chst1. ensuring species-specific recognition. It enables detection and localization of Chst1 expression in zebrafish tissues via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers employ this antibody to investigate Chst1's functional roles in developmental biology, ECM dynamics, and disease models. Its application has advanced understanding of how sulfation patterns influence cellular interactions, morphogen gradients, and tissue homeostasis in vivo. Validation often includes knockout controls to confirm specificity. This antibody is a key resource in zebrafish studies exploring GAG biology and its implications for vertebrate development and human pathologies.
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