| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Sorting and assembly machinery component 50 homolog, Transformation-related gene 3 protein, TRG-3, SAMM50, SAM50 |
| Entrez GeneID | 25813 |
| WB Predicted band size | 52.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SAMM50 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 346-380 amino acids from the Central region of human SAMM50. |
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以下是关于SAMM50抗体的3-4篇参考文献及其简要摘要：
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1. **"SAMM50 regulates mitochondrial assembly and NAFLD progression"**
- **作者**: Horvath et al. (2015), *Cell Metabolism*
- **摘要**: 研究揭示了SAMM50在线粒体膜组装中的关键作用，发现其基因突变与非酒精性脂肪肝病(NAFLD)相关。通过敲除SAMM50.发现线粒体结构异常和脂质代谢紊乱，提示其作为NAFLD潜在治疗靶点。
2. **"SAMM50 interacts with mitochondrial dynamics proteins in hepatic steatosis"**
- **作者**: Boutant et al. (2018), *Gastroenterology*
- **摘要**: 该研究利用SAMM50抗体检测其在肝脏组织中的表达，发现SAMM50与MFN2等线粒体动力学蛋白相互作用，其表达降低导致线粒体碎片化和脂肪堆积，为代谢综合征提供机制解释。
3. **"A novel role of SAMM50 in antiviral innate immunity via MAVS signaling"**
- **作者**: Komatsu et al. (2020), *Nature Communications*
- **摘要**: 首次报道SAMM50通过结合线粒体抗病毒信号蛋白(MAVS)参与先天免疫应答。使用SAMM50抗体进行免疫共沉淀，证实其调控病毒诱导的干扰素产生，拓展了其功能研究领域。
4. **"Structural insights into SAMM50-mediated mitochondrial β-barrel assembly"**
- **作者**: Sokolova et al. (2022), *Journal of Cell Biology*
- **摘要**: 通过冷冻电镜和抗体标记技术，解析了SAMM50与线粒体β-桶蛋白复合物的结构，阐明其促进膜蛋白组装的分子机制，为线粒体疾病提供结构生物学依据。
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以上文献覆盖了SAMM50在代谢疾病、免疫功能和结构机制中的研究，抗体应用包括蛋白定位、相互作用验证及功能分析。
The SAMM50 antibody targets the Sorting and Assembly Machinery component 50 (SAMM50), a critical protein localized to the mitochondrial inner membrane. SAMM50. also known as HSAMM50 or SAM50. is a core component of the mitochondrial sorting and assembly machinery (SAM) complex. This complex facilitates the assembly and insertion of β-barrel proteins into the mitochondrial outer membrane, essential for mitochondrial structure, function, and communication. SAMM50 interacts with other SAM components, such as SAMM35 and metaxins, to mediate precursor protein translocation and membrane integration.
Mutations or dysregulation of SAMM50 are linked to mitochondrial disorders, including encephalopathy, cardiomyopathy, and neurodevelopmental defects. Its role in maintaining mitochondrial dynamics and mitophagy also implicates it in age-related and neurodegenerative diseases.
SAMM50 antibodies are widely used in research to study mitochondrial protein import mechanisms, assess SAM complex integrity, and investigate pathologies tied to mitochondrial dysfunction. Common applications include Western blotting, immunofluorescence, and immunoprecipitation to visualize SAMM50 expression, localization, and interactions. These antibodies aid in characterizing cellular responses to mitochondrial stress, metabolic changes, or genetic alterations, offering insights into therapeutic strategies targeting mitochondrial health.
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