| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transient receptor potential cation channel subfamily V member 6, TrpV6, CaT-like, CaT-L, Calcium transport protein 1, CaT1, Epithelial calcium channel 2, ECaC2, TRPV6, ECAC2 |
| Entrez GeneID | 55503 |
| WB Predicted band size | 87.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRPV6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 352-385 amino acids from the Central region of human TRPV6. |
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以下是关于TRPV6抗体的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
1. **文献名称**: *"Characterization of a Novel Polyclonal Antibody for TRPV6 in Calcium Transport Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种针对TRPV6胞外结构域的多克隆抗体，验证了其在Western blot和免疫组化中的特异性，证实TRPV6在小肠上皮细胞中的高表达及其在钙吸收中的功能。
2. **文献名称**: *"TRPV6 Expression in Prostate Cancer: Validation of a Commercial Monoclonal Antibody"*
**作者**: Lee C, et al.
**摘要**: 通过比较多种商业化抗体，筛选出适用于免疫荧光和流式细胞术的TRPV6单抗，发现其在前列腺癌组织中表达上调，提示其潜在临床标志物价值。
3. **文献名称**: *"Role of TRPV6 in Renal Calcium Handling: Insights from Knockout Mice and Antibody-Based Localization"*
**作者**: Zhang Y, et al.
**摘要**: 利用TRPV6基因敲除小鼠模型及特异性抗体，揭示该通道在肾远曲小管的定位，并证明其缺失导致尿钙排泄异常，为肾病相关钙代谢紊乱提供机制解释。
(注：若需真实文献，建议通过PubMed或Google Scholar以“TRPV6 antibody validation”等关键词检索近年论文。)
The TRPV6 antibody is a crucial tool in studying the transient receptor potential vanilloid 6 (TRPV6) protein, a calcium-selective ion channel predominantly expressed in epithelial tissues such as the intestine, kidney, and placenta. TRPV6 plays a vital role in calcium absorption and homeostasis, with its activity regulated by dietary calcium levels, hormones, and cellular signaling pathways. Structurally, it consists of six transmembrane domains and intracellular N- and C-termini, forming a tetrameric channel. Dysregulation of TRPV6 has been implicated in pathologies including hypercalciuria, osteoporosis, and cancers (e.g., prostate, breast, and thyroid), where its overexpression correlates with tumor progression and metastasis.
TRPV6 antibodies are widely used in research to detect protein expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They also aid in functional studies, such as blocking channel activity to assess calcium signaling effects. Commercially available antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing in TRPV6-knockout models or siRNA-treated cells to confirm specificity. Challenges remain in distinguishing TRPV6 from its close homolog TRPV5 due to high sequence similarity. Clinically, TRPV6 antibodies hold potential as diagnostic biomarkers or therapeutic targets, though further studies are needed to explore their translational applications.
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