| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger and SCAN domain-containing protein 5B, ZSCAN5B |
| Entrez GeneID | 342933 |
| WB Predicted band size | 55.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZSCAN5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 403-431 amino acids from the C-terminal region of human ZSCAN5B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZSCAN5B抗体的示例参考文献(注：以下内容为示例性虚构，实际文献需根据具体研究查询)：
1. **文献名称**：*ZSCAN5B regulates pluripotency in human embryonic stem cells through epigenetic modulation*
**作者**：Chen L, et al.
**摘要**：本研究利用ZSCAN5B特异性抗体进行染色质免疫沉淀(ChIP-seq)，揭示了ZSCAN5B通过结合多能性相关基因启动子区域维持胚胎干细胞多能性，并影响组蛋白修饰。
2. **文献名称**：*Characterization of ZSCAN5B antibody specificity in cancer cell lines*
**作者**：Wang Y, et al.
**摘要**：通过Western blot和免疫荧光验证ZSCAN5B抗体的特异性，发现其在多种癌细胞系中高表达，且与肿瘤增殖相关信号通路存在关联。
3. **文献名称**：*ZSCAN5B interacts with telomeric repeats and safeguards chromosome stability*
**作者**：Smith J, et al.
**摘要**：使用ZSCAN5B抗体进行免疫共沉淀(Co-IP)实验，证实其与端粒重复结合蛋白相互作用，提示其在染色体稳定性和细胞衰老中的作用。
4. **文献名称**：*Developmental expression profile of ZSCAN5B in mouse embryogenesis*
**作者**：Tanaka K, et al.
**摘要**：通过免疫组化(IHC)结合ZSCAN5B抗体，系统分析了该蛋白在小鼠胚胎发育不同阶段的表达模式，发现其在神经前体细胞中显著富集。
建议通过PubMed或Google Scholar以“ZSCAN5B antibody”为关键词检索真实文献。
The ZSCAN5B antibody is a tool used to detect ZSCAN5B (Zinc Finger and SCAN Domain-Containing Protein 5B), a protein encoded by the ZSCAN5B gene located on human chromosome 19 (19q13.43). ZSCAN5B belongs to the KRAB-ZFP (Krüppel-associated box zinc finger protein) family, which plays roles in transcriptional regulation, chromatin remodeling, and genome stability. It is implicated in stem cell pluripotency, germ cell development, and early embryogenesis. Studies suggest ZSCAN5B may regulate telomere maintenance and chromosomal segregation, with abnormal expression linked to developmental disorders, including Down syndrome, and cancers such as testicular germ cell tumors.
ZSCAN5B antibodies, typically raised in rabbits or mice, are polyclonal or monoclonal reagents validated for applications like Western blotting (WB), immunofluorescence (IF), and immunohistochemistry (IHC). These antibodies target specific epitopes within the ZSCAN5B protein, aiding in studies of its expression patterns, subcellular localization (primarily nuclear), and interactions with chromatin or other regulatory factors. Researchers utilize ZSCAN5B antibodies to explore its role in maintaining pluripotency in embryonic stem cells, spermatogenesis, and potential contributions to oncogenesis. Recent work also highlights ZSCAN5B as a biomarker for germ cell lineage specification in vitro. Commercial ZSCAN5B antibodies often undergo validation via knockout/knockdown controls to ensure specificity, supporting their utility in developmental biology and cancer research.
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