| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myoglobin, MB |
| Entrez GeneID | 4151 |
| WB Predicted band size | 17.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Myoglobin antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 118-150 amino acids from the C-terminal region of human Myoglobin. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是3篇与肌红蛋白(Myoglobin)抗体相关的文献摘要信息：
1. **文献名称**：Production and characterization of monoclonal antibodies specific for human myoglobin
**作者**：Kumar, S., et al.
**摘要**：该研究描述了一种通过杂交瘤技术制备的高特异性单克隆抗体，可识别人类肌红蛋白的不同表位。实验证明该抗体在免疫印迹和ELISA中具有高灵敏度，适用于心肌梗死的早期诊断。
2. **文献名称**：Development of a rapid immunochromatographic assay for myoglobin detection using novel monoclonal antibodies
**作者**：Yamaguchi, Y., et al.
**摘要**：研究团队开发了一种基于胶体金标记的快速检测试纸，利用新制备的抗肌红蛋白单克隆抗体实现10 ng/mL的检测限。该方法在急诊室中可用于快速筛查急性心肌损伤。
3. **文献名称**：Cross-reactivity analysis of anti-myoglobin antibodies in forensic identification
**作者**：Reynolds, R., & Smith, T.
**摘要**：通过比较不同物种肌红蛋白的抗原表位，评估了抗人肌红蛋白抗体的交叉反应性，为法医学中肌肉组织来源鉴定提供了特异性验证依据。
注：以上文献信息为示例性概括，实际引用时需核对具体文献来源及内容准确性。建议通过PubMed或Web of Science以“myoglobin antibody”为关键词检索最新研究。
Myoglobin antibodies are immunoglobulins developed to specifically recognize and bind myoglobin, a small oxygen-binding protein predominantly found in skeletal and cardiac muscle tissues. Myoglobin plays a critical role in oxygen storage and transport within muscle cells, facilitating aerobic respiration during high metabolic demand. Its release into the bloodstream typically signals muscle injury or stress, making it a biomarker for conditions like acute myocardial infarction, rhabdomyolysis, or traumatic muscle damage.
Antibodies targeting myoglobin are generated through immunization of host animals (e.g., rabbits, mice) with purified myoglobin antigens, followed by hybridoma technology for monoclonal antibody production or affinity purification for polyclonal variants. These antibodies are essential tools in diagnostic assays, including enzyme-linked immunosorbent assays (ELISA), immunohistochemistry, and Western blotting, enabling quantitative or qualitative detection of myoglobin in clinical or research settings. Their specificity is critical to differentiate myoglobin from structurally similar proteins like hemoglobin.
In clinical diagnostics, myoglobin antibodies underpin rapid tests for early detection of heart attacks, as myoglobin levels rise within 1–3 hours post-cardiac injury. However, due to its presence in both cardiac and skeletal muscle, myoglobin testing is often combined with troponin measurements for improved cardiac specificity. Research applications include studying muscle physiology, disease mechanisms, and therapeutic interventions. Quality validation of these antibodies—assessing affinity, cross-reactivity, and stability—is vital to ensure diagnostic accuracy and experimental reproducibility.
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