| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cell cycle checkpoint control protein RAD9B, DNA repair exonuclease rad9 homolog B, hRAD9B, RAD9B |
| Entrez GeneID | 144715 |
| WB Predicted band size | 47.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAD9B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 394-428 amino acids from the C-terminal region of human RAD9B. |
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以下是关于RAD9B抗体的示例参考文献(均为假设性示例，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"Development of a High-Specificity RAD9B Antibody for DNA Damage Response Studies"*
**作者**: Tanaka K, et al.
**摘要**: 描述了针对人RAD9B蛋白的单克隆抗体的开发，验证其特异性(通过Western blot和免疫荧光)，并用于研究RAD9B在电离辐射后DNA损伤修复中的定位变化。
2. **文献名称**: *"RAD9B Expression in Ovarian Cancer: Validation of a Novel Immunohistochemical Marker"*
**作者**: Chen L, et al.
**摘要**: 报道了一种新型RAD9B抗体的临床验证，用于免疫组化检测卵巢癌组织中RAD9B的过表达，并探讨其与患者预后的相关性。
3. **文献名称**: *"Functional Analysis of RAD9B in Cell Cycle Checkpoints Using Antibody-Mediated Knockdown"*
**作者**: Müller S, et al.
**摘要**: 利用RAD9B特异性抗体进行功能阻断实验，证明RAD9B在G2/M期检查点调控中的作用，及其与ATR激酶的相互作用机制。
4. **文献名称**: *"Comparative Study of RAD9A and RAD9B Antibodies in Genomic Stability Assays"*
**作者**: Park J, et al.
**摘要**: 比较了RAD9A和RAD9B抗体的交叉反应性，验证了RAD9B抗体的亚型特异性，并应用于检测不同癌症细胞系中RAD9B的蛋白稳定性差异。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以关键词“RAD9B antibody”、“RAD9B antibody validation”或“RAD9B immunohistochemistry”检索。
The RAD9B antibody is a crucial tool for studying the RAD9 homolog B (RAD9B) protein, a member of the RAD9 family involved in DNA damage response and repair mechanisms. RAD9B shares structural homology with RAD9A, both forming the 9-1-1 (RAD9-RAD1-HUS1) complex, which acts as a sliding clamp to stabilize stalled replication forks and recruit repair proteins during genotoxic stress. Unlike RAD9A, RAD9B is primarily expressed in germ cells and plays distinct roles in meiosis, spermatogenesis, and oocyte development. It also participates in cell cycle checkpoints, apoptosis regulation, and maintaining genomic stability, with dysregulation linked to infertility and cancers such as prostate and ovarian tumors.
RAD9B antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interactions. Researchers employ these antibodies to explore RAD9B’s tissue-specific functions, its response to DNA-damaging agents, and its potential as a biomarker or therapeutic target. Notably, RAD9B antibodies have helped identify its cytoplasmic role in regulating apoptosis via interactions with BCL-2 family proteins, beyond its nuclear DNA repair functions.
When using RAD9B antibodies, validating specificity via knockout controls is essential due to potential cross-reactivity with RAD9A. Commercial antibodies often vary in performance across applications, necessitating optimization. Studies leveraging these reagents continue to unravel RAD9B’s dual roles in genome integrity and disease, highlighting its importance in reproductive and cancer biology.
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