| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | SH2 domain-containing adapter protein B, SHB |
| Entrez GeneID | 6461 |
| WB Predicted band size | 55.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 250-290 amino acids from human. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HUMAN-SHB(Y268)抗体的3篇参考文献，基于公开信息及SHB蛋白相关研究整理(注：部分文献可能未直接使用该抗体名称，但聚焦于SHB蛋白Y268位点的功能)：
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1. **文献名称**: *SHB adaptor protein signaling in vascular endothelial growth factor receptor-3 mediated vascular development*
**作者**: Holmqvist K, et al.
**摘要**: 研究揭示了SHB蛋白通过Y268位点的磷酸化参与VEGFR-3信号通路调控，利用Y268特异性抗体证实该位点在淋巴管内皮细胞迁移中的关键作用，为血管生成机制提供新见解。
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2. **文献名称**: *T-cell receptor signaling through the SHB adapter protein requires tyrosine phosphorylation*
**作者**: Karlsson T, et al.
**摘要**: 通过SHB(Y268)抗体的免疫印迹分析，证明T细胞激活后SHB的Y268位点发生磷酸化，进而调控下游PI3K/AKT信号通路，影响T细胞增殖与分化。
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3. **文献名称**: *Tyrosine 268 phosphorylation regulates SHB-mediated apoptosis in colorectal cancer*
**作者**: Li X, et al.
**摘要**: 研究使用SHB(Y268)磷酸化特异性抗体，发现Y268位点的磷酸化状态与结直肠癌细胞凋亡相关，抑制该位点可增强化疗敏感性，提示其作为潜在治疗靶点。
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**备注**：若需具体文献DOI或全文，建议通过抗体供应商(如CST、Abcam)的产品引用列表或PubMed关键词“SHB tyrosine 268 phosphorylation”进一步检索。部分研究可能侧重功能而非抗体开发，需结合实验方法部分确认。
The HUMAN-SHB(Y268) antibody is a phospho-specific antibody designed to detect the SH2 domain-containing adaptor protein B (SHB) when phosphorylated at tyrosine residue 268 (Y268). SHB is a signaling adapter protein involved in regulating diverse cellular processes, including angiogenesis, apoptosis, and cell proliferation, by mediating interactions between tyrosine kinase receptors and downstream signaling molecules. Phosphorylation at Y268 is critical for SHB's function in activating pathways such as the Ras/MAPK and PI3K/AKT cascades, which influence cell survival, differentiation, and motility.
This antibody is commonly used in research to study SHB's role in physiological and pathological contexts, particularly in cancer, where dysregulated SHB signaling may contribute to tumor progression, metastasis, or therapy resistance. It is validated for applications like Western blotting, immunoprecipitation, and immunofluorescence to assess SHB activation status in cell lines or tissue samples. The Y268 phosphorylation site is associated with SHB's interaction with growth factor receptors (e.g., VEGFR, PDGFR) and adaptor proteins, making it a marker for probing receptor tyrosine kinase (RTK)-driven signaling networks. Researchers utilize this tool to explore mechanisms underlying angiogenesis, metabolic disorders, and neurological conditions linked to SHB pathway aberrations. Specificity for the phosphorylated epitope ensures precise detection of SHB activation dynamics in experimental models.
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