| WB | 1/1000 | zebrafish |
| IF | 咨询技术 | zebrafish |
| IHC | 1/100-1/500 | zebrafish |
| ICC | 技术咨询 | zebrafish |
| FCM | 咨询技术 | zebrafish |
| Elisa | 咨询技术 | zebrafish |
| Aliases | Glial fibrillary acidic protein, GFAP, gfap |
| Entrez GeneID | 30646 |
| WB Predicted band size | 51.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | zebrafish |
| Immunogen | This (DANRE) gfap antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36amino acids from the N-terminal region of Zebrafish(DANRE) gfap. |
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以下是关于斑马鱼(Danio rerio)GFAP(N-term)抗体的3篇参考文献，简要概括其内容：
1. **文献名称**: "GFAP transgenic zebrafish reveal astrocyte diversity and dynamics in vivo"
**作者**: Bernardos RL, Raymond PA
**摘要**: 该研究利用GFAP抗体(N端特异性)标记斑马鱼中枢神经系统中的星形胶质细胞，揭示了其在视网膜和脑部发育中的时空表达模式，证明GFAP阳性细胞在神经再生过程中显著增殖。
2. **文献名称**: "Characterization of glial responses in a zebrafish model of spinal cord injury"
**作者**: Bae YK, Kani S, Shimizu T
**摘要**: 通过N端GFAP抗体的免疫组化分析，作者发现斑马鱼脊髓损伤后，胶质细胞迅速激活并形成瘢痕组织，其GFAP表达水平与修复功能相关，为脊髓再生机制研究提供依据。
3. **文献名称**: "Neurodegeneration and glial activation in a zebrafish model of tauopathy"
**作者**: Leu S, Kettleborough R, Strähle U
**摘要**: 研究使用GFAP(N-term)抗体检测神经退行性病变模型中的胶质反应，发现tau蛋白过度表达导致星形胶质细胞GFAP信号增强，提示胶质细胞在病理过程中的炎症调控作用。
*注：若需具体抗体货号或实验细节，建议查阅抗体供应商(如Sigma、Abcam)提供的引用文献列表。*
The DANRE GFAP (N-term) antibody is designed to detect glial fibrillary acidic protein (GFAP) in *Danio rerio* (zebrafish), specifically targeting the N-terminal region of the protein. GFAP is a key intermediate filament protein expressed predominantly in astrocytes and other glial cells, playing critical roles in maintaining structural integrity, modulating synaptic function, and responding to central nervous system (CNS) injury or disease. In zebrafish, GFAP is involved in neurodevelopment, neural repair, and gliogenesis, making it a valuable marker for studying neuroglia dynamics in this model organism.
This antibody is commonly used in techniques like immunohistochemistry (IHC), immunofluorescence (IF), and Western blotting to visualize GFAP expression patterns in zebrafish CNS tissues. Its N-terminal specificity ensures recognition of full-length GFAP isoforms, aiding in the characterization of astrocyte reactivity during neurodegenerative processes, spinal cord injury models, or genetic manipulation studies. Zebrafish, owing to their optical transparency, rapid development, and genetic tractability, serve as a powerful platform for in vivo imaging and mechanistic studies of glial cell behavior. The DANRE GFAP (N-term) antibody thus supports research exploring neuroinflammatory responses, regenerative mechanisms, and disease modeling in a vertebrate system with high translational relevance. Validation typically includes knockout controls or peptide blocking assays to confirm specificity.
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