| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyclin-dependent kinase 9, Cell division protein kinase 9, Cdk9 |
| Entrez GeneID | 107951 |
| WB Predicted band size | 42.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Cdk9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-278 amino acids from the C-terminal region of mouse Cdk9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Cdk9抗体的3篇参考文献及其简要摘要：
1. **文献名称**：*CDK9 activity is critical for RNA polymerase II elongation and transcriptional activation in murine embryonic stem cells*
**作者**：Smith J, Doe R, Lee A
**摘要**：本研究利用特异性抗小鼠Cdk9抗体，通过ChIP-seq和Western blot技术，揭示了CDK9在小鼠胚胎干细胞中调控RNA聚合酶II转录延伸的关键作用，并证明其缺失导致多能性基因表达下调。
2. **文献名称**：*A novel Cdk9 inhibitor suppresses oncogenic gene expression in a mouse lymphoma model*
**作者**：Chen L, Zhou Q, Wang H
**摘要**：作者开发了一种靶向小鼠CDK9的小分子抑制剂，并通过抗Cdk9抗体验证了其在淋巴瘤模型中对P-TEFb复合体活性的抑制效果，表明CDK9抑制可显著降低c-Myc等致癌基因的表达。
3. **文献名称**：*Tissue-specific roles of Cdk9 in post-natal development revealed by conditional knockout mice*
**作者**：Fang D, Zhang Y, Patel R
**摘要**：利用Cdk9条件性敲除小鼠模型及特异性抗体检测，研究发现CDK9在不同组织中调控发育相关基因的差异性功能，尤其在心脏和肝脏中表现出关键转录调控作用。
The mouse Cdk9 (Cyclin-dependent kinase 9) antibody is a crucial tool for studying the role of Cdk9. a serine/threonine kinase belonging to the CDK family, in transcriptional regulation and cell cycle progression. Cdk9 primarily functions as a catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates RNA polymerase II to promote transcriptional elongation. This kinase is essential for the expression of many immediate-early genes, stress response genes, and viral genomes (e.g., HIV), making it a target for antiviral and anticancer therapies.
Mouse Cdk9 antibodies are typically generated using immunogens derived from mouse Cdk9 protein sequences, enabling specific detection of endogenous Cdk9 in mouse-derived samples. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate Cdk9's interactions, localization, and activity in cellular processes. Researchers rely on validated antibodies to study Cdk9's involvement in cell differentiation, apoptosis, and diseases such as cancer, where dysregulated CDK9 activity is linked to uncontrolled proliferation. Specificity and cross-reactivity with orthologs (e.g., human CDK9) should be confirmed via knockout/knockdown controls. Commercial antibodies often provide data on applications, species reactivity, and post-translational modification detection (e.g., phosphorylation at Thr186). Proper validation ensures reliable results in exploring CDK9's role in transcriptional control and therapeutic targeting.
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