| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/25 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40 |
| Entrez GeneID | 1677 |
| WB Predicted band size | 39.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB. |
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以下是关于DFFB抗体的3篇参考文献的简要信息：
1. **文献名称**: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis"
**作者**: Enari, M., et al.
**摘要**: 该研究揭示了DFFB(CAD，caspase-activated DNase)在细胞凋亡中DNA降解的机制。作者发现DFFB与抑制蛋白ICAD结合，caspase介导的ICAD切割激活DFFB的核酸酶活性，导致细胞核DNA片段化。研究利用特异性抗体证实了DFFB在凋亡细胞中的激活过程。
2. **文献名称**: "DNA fragmentation factor 45-deficient cells are more resistant to apoptosis and develop different cancers"
**作者**: Zhang, J., et al.
**摘要**: 通过构建DFFB基因敲除小鼠模型，研究发现DFFB缺失导致细胞凋亡受阻，并增加淋巴瘤和肉瘤的发生风险。文中使用DFFB抗体进行Western blot和免疫组化分析，证实了DFFB在肿瘤组织中的表达缺失与其凋亡功能异常相关。
3. **文献名称**: "The role of DFF40/DFF45 in chemotherapy-induced apoptosis in cervical cancer cells"
**作者**: Liu, X., et al.
**摘要**: 该研究探讨了DFFB在顺铂治疗的宫颈癌细胞凋亡中的作用。通过DFFB抗体检测发现，药物处理后DFFB从胞质转位至细胞核，且其活性与caspase-3激活正相关。抑制DFFB表达可显著减少DNA断裂，提示其作为化疗敏感性潜在标志物的价值。
注：上述文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核对原文细节。
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