| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KiSS-1 receptor, KiSS-1R, G-protein coupled receptor 54, G-protein coupled receptor OT7T175, hOT7T175, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor, KISS1R, AXOR12, GPR54 |
| Entrez GeneID | 84634 |
| WB Predicted band size | 42.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This KISS1R antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-263 amino acids from the Central region of human KISS1R. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于KISS1R抗体的3篇参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**: *"Characterization of KISS1R antibody specificity in human hypothalamic tissues"*
**作者**: Smith A, et al.
**摘要**: 本研究通过免疫组化验证了一种兔源多克隆KISS1R抗体的特异性，发现其在人下丘脑神经元中特异性标记KISS1R阳性细胞，并证实其与生殖激素调控相关。抗体经肽段阻断实验验证无交叉反应。
2. **文献名称**: *"Role of KISS1R in ovarian follicle development: Insights from antibody-based localization"*
**作者**: Chen L, et al.
**摘要**: 利用小鼠卵巢组织，通过Western blot和免疫荧光技术分析KISS1R蛋白表达，发现抗体(货号#AB123. 小鼠单克隆)可特异性识别卵泡颗粒细胞中的KISS1R，提示其在卵泡成熟中的作用。
3. **文献名称**: *"Development and validation of a novel KISS1R antibody for primate models"*
**作者**: Tanaka K, et al.
**摘要**: 研究报道了一种新型猴源KISS1R多克隆抗体的开发，通过ELISA和免疫沉淀实验验证其高亲和力，并成功应用于猕猴脑组织的KISS1R表达谱分析，支持跨物种比较研究。
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注：以上文献为示例，实际文献需通过PubMed或学术数据库检索(关键词如KISS1R antibody、GPR54 antibody validation)。建议结合具体实验需求选择经过验证的商业化抗体相关文献(如CiteAb或供应商提供的参考文献)。
KISS1R (Kisspeptin Receptor), also known as GPR54. is a G protein-coupled receptor that binds kisspeptins, a family of neuropeptides encoded by the KISS1 gene. Discovered in the early 2000s, KISS1R gained prominence for its critical role in regulating reproductive physiology, particularly in initiating puberty and modulating gonadotropin-releasing hormone (GnRH) secretion. It activates signaling pathways like phospholipase C (PLC) and mitogen-activated protein kinase (MAPK), influencing cell proliferation, migration, and apoptosis.
KISS1R antibodies are essential tools for studying receptor expression, localization, and function in research. They enable detection of KISS1R in tissues (e.g., brain, placenta, pancreas) via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of KISS1R signaling is linked to reproductive disorders (e.g., hypogonadism, precocious puberty) and cancer progression, as KISS1 acts as a metastasis suppressor. Antibodies targeting specific epitopes (extracellular or intracellular domains) help investigate receptor trafficking, ligand interactions, and downstream effects.
Commercial KISS1R antibodies are typically raised in rabbits or mice, validated for specificity in knockout models. Challenges include cross-reactivity with related GPCRs, necessitating careful validation. Ongoing research explores therapeutic targeting of KISS1R for fertility treatments or cancer, amplifying the antibody's utility in both basic and translational studies.
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