| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor 21, TCF-21, Capsulin, Class A basic helix-loop-helix protein 23, bHLHa23, Epicardin, Podocyte-expressed 1, Pod-1, TCF21, BHLHA23, POD1 |
| Entrez GeneID | 6943 |
| WB Predicted band size | 19.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于TCF21抗体的参考文献摘要，信息基于模拟生成(非真实文献)：
1. **文献名称**: *TCF21 regulates coronary artery development through epithelial-mesenchymal transition*
**作者**: Smith J, et al.
**摘要**: 本研究利用TCF21抗体进行免疫组化分析，发现TCF21在胚胎期冠状动脉内皮细胞中高表达，通过调控EMT相关基因参与血管形成，证实其缺失导致小鼠心脏发育异常。
2. **文献名称**: *TCF21 as a tumor suppressor in lung adenocarcinoma: Antibody-based biomarker validation*
**作者**: Chen L, et al.
**摘要**: 通过Western blot和免疫荧光技术，研究者发现TCF21在肺癌组织中表达下调，其抗体检测结果与患者预后显著相关，提示TCF21可作为潜在诊断标志物。
3. **文献名称**: *Chromatin profiling reveals TCF21-mediated transcriptional networks in renal fibrosis*
**作者**: Wang Y, et al.
**摘要**: 结合ChIP-seq(使用TCF21抗体)和RNA-seq技术，揭示了TCF21在肾脏纤维化中通过调控TGF-β通路基因抑制成纤维细胞活化，为治疗提供新靶点。
4. **文献名称**: *Antibody validation for TCF21 in human pluripotent stem cell differentiation*
**作者**: Kim S, et al.
**摘要**: 该研究系统验证了多种TCF21抗体的特异性，确认其在人干细胞向心肌细胞分化过程中的表达动态，为体外模型研究提供可靠工具。
(注：以上文献为示例，实际引用需查询PubMed等数据库获取真实信息。)
**Background of TCF21 Antibody**
The TCF21 (Transcription Factor 21) antibody is a tool used to detect and study the TCF21 protein, a member of the basic helix-loop-helix (bHLH) transcription factor family. TCF21. also known as Pod1 or Capsulin, plays critical roles in cell differentiation, organogenesis, and tissue development, particularly in the heart, kidneys, and lungs. It regulates gene expression by binding to E-box DNA motifs, influencing cell fate decisions during embryogenesis and maintaining tissue homeostasis in adults.
In research, TCF21 antibodies are widely employed in techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence (IF) to localize and quantify TCF21 expression in tissues or cell lines. Studies highlight its involvement in diseases such as cancer, fibrosis, and cardiovascular disorders. For example, TCF21 acts as a tumor suppressor in multiple cancers, including lung and breast cancers, where its downregulation correlates with tumor progression and poor prognosis. In fibrosis, TCF21 modulates epithelial-mesenchymal transitions (EMT), impacting tissue repair and scarring.
The development and validation of TCF21 antibodies are crucial for elucidating its molecular mechanisms, interactions, and therapeutic potential. Specificity and sensitivity of these antibodies are rigorously tested to ensure accurate detection, aiding advancements in developmental biology, oncology, and regenerative medicine research.
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