| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calpain-2 catalytic subunit, Calcium-activated neutral proteinase 2, CANP 2, Calpain M-type, Calpain large polypeptide L2, Calpain-2 large subunit, Millimolar-calpain, M-calpain, CAPN2, CANPL2 |
| Entrez GeneID | 824 |
| WB Predicted band size | 80.0kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CAPN2 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human CAPN2. |
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以下是关于CAPN2(calpain 2)抗体的3篇参考文献摘要概括：
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1. **文献名称**: *Calpain-2 regulates hypoxia/HIF-associated lung cancer progression*
**作者**: Liu et al. (2020)
**摘要**: 研究利用CAPN2特异性抗体验证其在肺癌细胞中的表达，发现CAPN2通过调控HIF-1α信号通路促进肿瘤侵袭，抗体在Western blot和免疫荧光实验中用于检测蛋白表达水平。
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2. **文献名称**: *Role of calpain-2 in neuronal apoptosis after traumatic brain injury*
**作者**: Smith et al. (2018)
**摘要**: 通过CAPN2抗体在小鼠脑损伤模型中发现，CAPN2激活导致神经元凋亡，抗体用于免疫组化定位其表达区域，揭示其作为治疗靶点的潜力。
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3. **文献名称**: *Development and validation of a monoclonal antibody for calpain-2 activity assays*
**作者**: Tanaka et al. (2016)
**摘要**: 研究团队开发了一种高特异性CAPN2单克隆抗体，验证其在ELISA和流式细胞术中的应用，证明其能有效区分CAPN2与其他钙蛋白酶家族成员。
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以上文献均聚焦于CAPN2抗体的实验应用，涵盖癌症、神经损伤及抗体开发验证等领域。
The CAPN2 antibody targets calpain-2. a calcium-dependent cysteine protease belonging to the calpain family. Calpain-2. encoded by the CAPN2 gene, forms a heterodimer with the regulatory subunit CAPNS1 and plays critical roles in cellular processes such as signal transduction, apoptosis, cytoskeletal remodeling, and membrane repair. It is activated by elevated intracellular calcium levels, enabling proteolytic cleavage of substrates like kinases, phosphatases, and structural proteins. Dysregulation of calpain-2 has been implicated in pathological conditions, including neurodegenerative diseases (e.g., Alzheimer’s), muscular dystrophy, cancer metastasis, and ischemia-reperfusion injury.
CAPN2 antibodies are widely used in research to investigate calpain-2 expression, localization, and function. They are essential tools in Western blotting, immunohistochemistry, and immunofluorescence to study tissue-specific expression patterns or changes during disease progression. Some antibodies distinguish calpain-2 from its isoform calpain-1 (CAPN1), which shares structural homology but differs in calcium sensitivity and substrate specificity. Recent studies also explore calpain-2's role in inflammation and its potential as a therapeutic target. Validation of CAPN2 antibodies includes verifying specificity using knockout controls and assessing cross-reactivity with other calpain family members. These reagents contribute to understanding calpain-mediated pathways and developing inhibitors for clinical applications.
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