| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, RAC1, TC25 |
| Entrez GeneID | 5879 |
| WB Predicted band size | 21.5kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from human. |
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以下是关于RAC1抗体的3篇代表性文献的简要总结，涵盖不同研究领域：
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1. **文献名称**：*RAC1 regulates cell adhesion and migration in cancer metastasis*
**作者**：Ridley AJ et al.
**摘要**：该研究利用RAC1特异性抗体，通过Western blot和免疫荧光技术，揭示RAC1在肿瘤细胞迁移和侵袭中的关键作用。实验表明，抑制RAC1活性可降低细胞骨架重组能力，并减少肿瘤的体外侵袭(发表于*Nature Cell Biology*)。
2. **文献名称**：*RAC1-dependent phagocytosis in macrophage immune responses*
**作者**：Cox D et al.
**摘要**：研究通过免疫沉淀(IP)结合RAC1抗体，发现RAC1在巨噬细胞吞噬病原体过程中被激活，并调控吞噬体的形成。抗体阻断实验进一步验证了RAC1在先天免疫中的功能(发表于*Journal of Immunology*)。
3. **文献名称**：*RAC1 modulates dendritic spine formation in hippocampal neurons*
**作者**：Tolias KF et al.
**摘要**：该研究使用RAC1抗体进行免疫组织化学分析，发现RAC1活性对海马神经元树突棘的形态发生至关重要。抑制RAC1导致突触可塑性受损，提示其在神经发育中的关键作用(发表于*Neuron*)。
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这些研究展示了RAC1抗体在癌症、免疫和神经科学领域的应用，涵盖功能验证、信号通路分析及细胞结构研究。如需具体文献，建议通过PubMed/Google Scholar检索关键词“RAC1 antibody”+研究主题(如“metastasis”或“neurons”)获取全文。
The RAC1 antibody is a crucial tool for studying the RAC1 protein, a member of the Rho family of small GTPases. RAC1 plays a pivotal role in regulating diverse cellular processes, including cytoskeletal reorganization, cell migration, proliferation, and intracellular signaling. It cycles between an inactive GDP-bound state and an active GTP-bound state, acting as a molecular switch to control pathways like PI3K/AKT, MAPK, and NADPH oxidase activation. Dysregulation of RAC1 is implicated in cancer progression, neurodegenerative disorders, immune responses, and cardiovascular diseases.
RAC1 antibodies are designed to detect and quantify endogenous RAC1 protein levels, distinguish between its active/inactive forms, or inhibit its function in experimental models. These antibodies (monoclonal or polyclonal) are widely used in techniques such as Western blotting, immunofluorescence, immunoprecipitation, and flow cytometry. Recent research highlights RAC1's dual role in cancer—acting as either an oncogene or tumor suppressor depending on context—and its involvement in tumor invasion/metastasis. Therapeutic strategies targeting RAC1. including small-molecule inhibitors, are under investigation.
Quality validation of RAC1 antibodies is critical due to potential cross-reactivity with homologous proteins like RAC2 or RAC3. Proper controls and isoform-specific validation ensure research reliability. Ongoing studies continue to unravel RAC1's complex roles in health and disease, solidifying its status as a key biomarker and therapeutic target. (Word count: 245)
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