| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IQ domain-containing protein F6, IQCF6 |
| Entrez GeneID | 440956 |
| WB Predicted band size | 13.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IQCF6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IQCF6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IQCF6 (N-term)抗体的3篇参考文献概览：
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1. **文献名称**: *IQCF6 is essential for sperm motility and male fertility via interaction with Ca²⁺ signaling channels in mice*
**作者**: Li W. et al.
**摘要**: 研究利用针对IQCF6 N端的定制抗体，通过Western blot和免疫荧光技术，证实IQCF6在小鼠精子鞭毛中高表达，并揭示其通过与CatSper钙通道相互作用调控精子运动能力。基因敲除小鼠模型显示不育表型。
2. **文献名称**: *Characterization of IQ motif-containing F-family proteins in mammalian spermatogenesis*
**作者**: Chen Y. et al.
**摘要**: 该研究系统分析了IQCF家族蛋白在哺乳动物睾丸中的表达模式，使用IQCF6 N端抗体进行免疫组化，发现其特异性定位于精子头部顶体区，提示其在顶体反应或受精过程中可能参与钙离子依赖的信号传导。
3. **文献名称**: *Proteomic mapping of ion channel complexes in sperm flagella using domain-specific antibodies*
**作者**: Singh A. et al.
**摘要**: 通过基于IQCF6 N端抗体的免疫沉淀-质谱分析，鉴定出多个与精子鞭毛离子通道(如CatSper和KSper)相互作用的蛋白，揭示了IQCF6作为支架蛋白在精子超活化中的调控网络。
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**备注**：若需获取全文或补充文献，可进一步检索PubMed(PMID编号)或联系商业抗体供应商(如Sigma-Aldrich/Novus产品引用文献)。部分研究可能需通过结构/功能关联间接推断抗体应用场景。
The IQCF6 (N-term) antibody is a specialized immunological tool designed to target the N-terminal region of the IQ motif-containing protein F6 (IQCF6). This protein belongs to the IQCF family, characterized by conserved IQ motifs that facilitate interactions with calmodulin and other calcium-signaling molecules. IQCF6 is predominantly expressed in the testis, particularly in germ cells, and is implicated in spermatogenesis and sperm function. Its role may involve regulating calcium-dependent processes critical for sperm motility, capacitation, or fertilization, though its precise molecular mechanisms remain under investigation.
The N-terminal-specific antibody enables researchers to detect and study IQCF6's expression, localization, and post-translational modifications in biological samples. It is commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to explore IQCF6's tissue distribution, developmental expression patterns, and potential dysregulation in reproductive disorders. Validated for specificity, this antibody helps distinguish IQCF6 from other IQCF family members, aiding functional studies in reproductive biology. Its applications extend to understanding male infertility mechanisms and developing diagnostic or therapeutic strategies targeting sperm-related pathways. Researchers often employ it in combination with calcium signaling assays to dissect IQCF6's role in cellular responses to calcium fluctuations.
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