| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ribonuclease inhibitor, Placental ribonuclease inhibitor, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, PRI, RNH |
| Entrez GeneID | 6050 |
| WB Predicted band size | 50.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from the C-terminal region of human RNH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RNH1抗体的模拟参考文献示例(仅供格式参考，建议通过学术数据库获取真实文献)：
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1. **标题**: *RNH1 Antibody Development for Detecting Oxidative Stress in Neurodegenerative Diseases*
**作者**: Smith, J.R., et al.
**摘要**: 本研究开发了一种高特异性RNH1多克隆抗体，用于检测阿尔茨海默病模型中RNH1的氧化修饰状态。通过免疫印迹和免疫组化验证，发现RNH1的异常表达与神经元氧化损伤呈正相关。
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2. **标题**: *Role of RNH1 in Cancer Progression: Validation by a Novel Monoclonal Antibody*
**作者**: Lee, H., & Zhang, Y.
**摘要**: 利用新研制的RNH1单克隆抗体，分析结直肠癌组织样本中RNH1的表达水平。结果表明，RNH1低表达与患者预后不良相关，提示其可能通过调控血管生成抑制肿瘤转移。
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3. **标题**: *Cross-Reactivity Analysis of Commercial RNH1 Antibodies Using Knockout Cell Models*
**作者**: García, M., et al.
**摘要**: 系统评估了5种市售RNH1抗体的特异性，发现仅两种抗体在RNH1基因敲除细胞系中无交叉反应。研究强调了抗体验证在RNA酶调控研究中的重要性。
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4. **标题**: *Structural Insights into RNH1-RNAse Complexes Revealed by Epitope-Specific Antibodies*
**作者**: Watanabe, K., et al.
**摘要**: 通过表位特异性抗体解析RNH1与RNAse的相互作用界面，发现其C端结构域在抑制RNA酶活性中的关键作用，为开发靶向疗法提供了新思路。
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**注意**：以上为模拟文献，实际文献需通过PubMed、Web of Science等平台检索(关键词如“RNH1 antibody”或“Ribonuclease inhibitor antibody”)。如需具体文献协助，请提供更多研究背景。
The RNH1 antibody is a crucial tool in molecular biology research, targeting Ribonuclease Inhibitor 1 (RNH1), a conserved leucine-rich repeat protein that binds and inhibits ribonucleases like RNase A family members. RNH1 plays vital roles in maintaining RNA integrity by blocking RNase activity, regulating RNA metabolism, and participating in cellular stress responses. It also interacts with angiogenin, modulating RNA interference and angiogenesis. Dysregulation of RNH1 has been implicated in cancer, neurodegenerative disorders (e.g., ALS), and autoimmune diseases, making its study biologically significant.
RNH1 antibodies, typically generated in rabbits or mice through recombinant protein immunization, enable the detection of endogenous RNH1 (~50 kDa) in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. These antibodies help map RNH1's subcellular localization (primarily cytoplasmic), expression patterns across tissues, and interactions with partner proteins. High-quality antibodies are validated using knockout cell lines or siRNA knockdown to confirm specificity. Recent studies utilize RNH1 antibodies to explore its dual role in cancer—as both a tumor suppressor (by stabilizing RNA) and a potential oncogenic factor (via stress granule modulation). Their applications extend to investigating RNH1's emerging functions in viral infection responses and epigenetic regulation.
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