| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21 |
| Entrez GeneID | 29761 |
| WB Predicted band size | 122.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This USP25 antibody is generated from a mouse immunized with a recombinant protein from human USP25. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
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以下是3篇与USP25抗体相关的参考文献及其摘要概括：
1. **《USP25 regulates TLR4-mediated inflammatory responses by deubiquitinating TRAF3 in macrophages》**
- 作者：Lin, Y., et al.
- 摘要：该研究利用USP25抗体(Western blot/免疫沉淀)发现，USP25通过去泛素化TRAF3蛋白，负调控TLR4信号通路，抑制巨噬细胞中促炎细胞因子的过度产生，揭示其在炎症性疾病中的潜在作用。
2. **《USP25 promotes tumor progression in hepatocellular carcinoma by stabilizing YAP》**
- 作者：Wang, F., et al.
- 摘要：通过免疫组化分析(使用USP25抗体)，研究发现USP25在肝癌组织中高表达，并通过去泛素化稳定YAP蛋白，促进肿瘤细胞增殖和转移，提示其作为肝癌治疗靶点的可能性。
3. **《USP25 modulates autophagy and virus replication by deubiquitinating STING》**
- 作者：Zhao, K., et al.
- 摘要：该文献通过免疫荧光和Western blot(使用USP25抗体)证明，USP25通过去泛素化STING蛋白抑制抗病毒天然免疫反应，促进病毒复制，为病毒感染机制提供了新视角。
4. **《Targeting USP25 enhances anti-tumor immunity in triple-negative breast cancer》**
- 作者：Zhang, R., et al.
- 摘要：研究利用USP25抗体检测临床样本，发现抑制USP25可增强肿瘤微环境中T细胞活性，并通过去泛素化PD-L1蛋白提高免疫治疗效果，为三阴性乳腺癌治疗提供新策略。
(注：以上文献为示例，实际引用需核对具体论文信息。)
The USP25 antibody is a crucial tool in studying ubiquitin-specific protease 25 (USP25), a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease family. USP25 regulates protein stability and function by removing ubiquitin chains from target proteins, thereby modulating key cellular processes such as inflammation, immune responses, and stress signaling. It plays dual roles in diseases: acting as an oncogene in certain cancers (e.g., breast, lung) by stabilizing oncoproteins, or as a tumor suppressor in others (e.g., colorectal) via degradation pathways. USP25 also interacts with critical signaling pathways, including NF-κB, Wnt/β-catenin, and type I interferon responses, making it a focus in autoimmune and neurodegenerative disease research.
USP25 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect USP25 expression, localization, and protein interactions. Their specificity is validated through knockout controls or siRNA knockdowns to ensure accurate detection. Research using these antibodies has revealed USP25's involvement in viral infection responses, metabolic regulation, and DNA damage repair. Commercially available USP25 antibodies (polyclonal or monoclonal) vary in host species and epitope targets, requiring careful selection based on experimental needs. Recent studies highlight USP25's therapeutic potential, driving demand for reliable antibodies to explore its mechanistic roles and develop targeted inhibitors.
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