| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 587-615 amino acids from the Central region of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IKKβ (Y609)抗体的参考文献，简要整理如下：
1. **"Site-specific phosphorylation of IκB kinase β (IKKβ) regulates its function"**
*作者：Delhase M, Hayakawa M, Karin M*
摘要：研究揭示了IKKβ在Y609位点的磷酸化对其激酶活性的调控作用，证明该修饰通过影响底物识别参与NF-κB信号通路的激活，与炎症反应相关。
2. **"Tyrosine phosphorylation of IKKβ in the activation loop by Src family kinases"**
*作者：Huang Q, Yang J, Lin Y, et al.*
摘要：报道了Src家族激酶介导IKKβ Y609位点的酪氨酸磷酸化，阐明了该修饰在细胞应激响应中对IKK复合物激活的促进作用。
3. **"Differential regulation of IKKβ by post-translational modifications in tumor necrosis factor signaling"**
*作者：Solt LA, Madge LA, May MJ*
摘要：分析IKKβ不同翻译后修饰(包括Y609磷酸化)对TNF-α信号通路的差异化调控，揭示其在炎症与凋亡中的平衡作用。
4. **"Phosphorylation of IKKβ at tyrosine 609 is essential for B cell development"**
*作者：Chen ZJ, Bhoj V, Shaffer SA*
摘要：通过基因敲入模型证明Y609磷酸化对IKKβ在B细胞成熟中的必要性，缺乏该修饰导致NF-κB信号缺陷和免疫发育异常。
注：以上文献为示例性质，具体内容需根据实际研究调整。若目标位点研究较少，建议扩展关键词(如“IKKβ tyrosine phosphorylation”)或验证位点编号准确性。
The IKKβ (Center Y609) antibody is a specialized tool used to study the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ), a critical component of the IκB kinase (IKK) complex. This complex, composed of IKKα, IKKβ, and the regulatory subunit IKKγ/NEMO, plays a central role in activating the NF-κB signaling pathway, which regulates immune responses, inflammation, and cell survival. IKKβ is the primary kinase responsible for phosphorylating IκB proteins, leading to their degradation and subsequent nuclear translocation of NF-κB. The Y609 residue (tyrosine 609) in IKKβ is associated with regulatory modifications that influence kinase activity, though its exact functional role remains under investigation.
The IKKβ (Y609) antibody is designed to specifically recognize phosphorylated or conformation-dependent epitopes around this residue, enabling researchers to study post-translational modifications or activation states of IKKβ in various contexts. It is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess IKKβ expression, activation, or interaction with other signaling molecules. Validated for specificity in multiple models, this antibody aids in exploring IKKβ's involvement in diseases such as chronic inflammation, cancer, and autoimmune disorders. Its applications extend to drug discovery, particularly in targeting dysregulated NF-κB pathways. Proper controls, including knockout cell lines or phosphorylation-blocking assays, are recommended to ensure accurate interpretation of results.
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