关键信息
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基因名
mrkD
- 应用
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种属
Klebsiella pneumoniae
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表达系统
E. coli
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标签
N- His & C- Myc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P21648
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表达区间
19-321aa
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分子量
39.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
The study of the MrkD recombinant protein has garnered significant interest due to its potential role in understanding bacterial adhesion and biofilm formation, particularly in uropathogenic strains of Escherichia coli. MrkD is a critical adhesin protein associated with type 3 fimbriae, which facilitate the attachment of bacteria to host tissues and surfaces, thereby contributing to the pathogenicity of urinary tract infections. Research into MrkD not only helps unveil the mechanisms underlying bacterial colonization but also offers insights into the development of therapeutic strategies, such as vaccines or inhibitors that could disrupt the adhesion process. Furthermore, the recombinant expression of MrkD allows for the generation of sufficient quantities of the protein for advanced studies, enabling researchers to analyze its structure, function, and interaction with host cells in detail. As antibiotic resistance continues to rise, understanding proteins like MrkD is essential for developing novel approaches to combat bacterial infections and enhance our overall grasp of bacterial pathogenesis.
