| WB | DB: 1/500 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Receptor tyrosine-protein kinase erbB-2, Epidermal growth factor receptor-related protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, p185neu, CD340, Erbb2, Neu |
| WB Predicted band size | 138.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This rat ERBB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1168 of rat ERBB2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-rat ERBB2 (T1168)抗体的参考文献示例(注：由于该位点特异性较高，部分文献可能涉及人源或类似磷酸化位点的研究)：
1. **文献名称**: "ERBB2 phosphorylation at tyrosine 1248 in rat glioblastoma cells mediates Src-dependent cell invasion"
**作者**: Smith et al.
**摘要**: 研究通过Phospho-rat ERBB2 (T1168)抗体检测大鼠胶质母细胞瘤模型中ERBB2在Tyr1168(对应人Y1248)的磷酸化，发现其通过激活Src激酶促进肿瘤细胞侵袭。
2. **文献名称**: "Phosphorylation-dependent regulation of ERBB2 signaling in mammary epithelial cells"
**作者**: Johnson & Lee
**摘要**: 使用Phospho-rat ERBB2 (T1168)抗体分析大鼠乳腺上皮细胞中ERBB2信号通路，发现T1168磷酸化与受体二聚化及下游MAPK通路激活相关。
3. **文献名称**: "Targeting ERBB2 phosphorylation in rat models of cardiac hypertrophy"
**作者**: Chen et al.
**摘要**: 通过该抗体证实心脏肥大模型中ERBB2在T1168位点的异常磷酸化，并探索其作为治疗靶点的潜力。
4. **文献名称**: "Comparative analysis of ERBB2 phosphorylation sites across species"
**作者**: Martinez et al.
**摘要**: 对比大鼠(T1168)与人(Y1248)ERBB2磷酸化位点的保守性，验证抗体特异性及跨物种研究中的适用性。
**注意**：以上文献为示例，实际研究中需结合具体实验需求检索真实文献(可通过PubMed或抗体供应商提供的参考文献链接)。
The Phospho-rat ERBB2 (T1168) antibody is a specialized immunoassay tool designed to detect the phosphorylated form of rat ERBB2 (also known as HER2/Neu) at threonine residue 1168. ERBB2. a member of the epidermal growth factor receptor (EGFR) tyrosine kinase family, plays a critical role in regulating cell proliferation, differentiation, and survival. Its activation typically occurs via ligand-induced heterodimerization with other EGFR family members, leading to autophosphorylation of specific tyrosine/threonine residues in the intracellular domain. Phosphorylation at T1168 is associated with receptor activation and downstream signaling pathways, including MAPK/ERK and PI3K/AKT, which are often dysregulated in cancers.
This antibody is particularly useful in studies investigating ERBB2 signaling dynamics in rat models, enabling researchers to assess receptor activation status in response to stimuli or therapeutic interventions. It is commonly applied in techniques like Western blotting, immunohistochemistry, or immunofluorescence to quantify phosphorylation levels in tissue or cell lysates. Given ERBB2's oncogenic potential when overexpressed or hyperactivated, this antibody also supports cancer research, especially in exploring mechanisms of tumor progression, metastasis, and resistance to targeted therapies like trastuzumab. Specificity for the phosphorylated T1168 epitope ensures minimal cross-reactivity with non-phosphorylated ERBB2 or other EGFR family members, making it a precise tool for mechanistic studies.
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