| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MAP1LC3A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human MAP1LC3A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-MAP1LC3A(S12)抗体的模拟参考文献示例(注：文献为构造示例，建议通过学术数据库检索真实研究)：
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1. **"ULK1-mediated phosphorylation of MAP1LC3A at Ser12 regulates autophagosome maturation"**
*Authors: Lee, J. et al.*
摘要：该研究报道ULK1激酶在自噬起始阶段磷酸化LC3A的Ser12位点，通过特异性抗体验证该修饰促进自噬体与溶酶体融合，并影响饥饿条件下细胞存活。
2. **"A phospho-specific antibody reveals dynamic LC3A Ser12 phosphorylation during mitophagy"**
*Authors: Zhang, Y. et al.*
摘要：开发了一种高特异性Phospho-LC3A(S12)抗体，证明线粒体自噬过程中该位点的磷酸化受PINK1调控，并与Parkin协同作用清除受损线粒体。
3. **"Phosphorylation of LC3A at Ser12 modulates its interaction with selective autophagy receptors"**
*Authors: Nguyen, T. et al.*
摘要：利用S12磷酸化抗体，发现该修饰增强LC3A与OPTN的结合能力，提示其在选择性自噬(如异源自噬)中的调控作用，并影响病原体清除效率。
4. **"Validation of a novel phospho-MAP1LC3A (Ser12) antibody for monitoring autophagy in vivo"**
*Authors: Sato, M. et al.*
摘要：系统验证了该抗体的特异性及在组织样本中的应用，证明S12磷酸化水平与小鼠肝脏自噬激活程度正相关，可作为自噬动态标志物。
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建议通过PubMed或Web of Science检索关键词“MAP1LC3A phosphorylation Ser12”或“LC3A S12 phospho-antibody”获取最新研究文献。
The Phospho-MAP1LC3A (S12) antibody is a specialized tool used to study autophagy, a critical cellular recycling process. MAP1LC3A (microtubule-associated protein 1 light chain 3 alpha), also known as LC3A, is a key marker of autophagosomes—vesicles that engulf and degrade cellular debris. During autophagy, cytosolic LC3A (LC3-I) is conjugated to phosphatidylethanolamine, forming LC3-II, which integrates into autophagosomal membranes. Phosphorylation at serine 12 (S12) is a post-translational modification implicated in regulating LC3A’s function, though its exact role remains under investigation. Some studies suggest this modification may influence LC3A’s interaction with other autophagy-related proteins or its stability.
The Phospho-MAP1LC3A(S12) antibody specifically detects LC3A when phosphorylated at S12. enabling researchers to study dynamic changes in autophagy signaling. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to monitor autophagic activity in response to stressors (e.g., nutrient deprivation, hypoxia) or disease states (e.g., cancer, neurodegeneration). Validating its specificity is crucial, often achieved using LC3A-knockout models or phosphatase-treated lysates. This antibody has advanced understanding of autophagy dysregulation in pathologies and therapeutic interventions targeting autophagy pathways. However, interpretation requires caution, as LC3 phosphorylation can vary by cell type and context.
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