| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S177 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-IKKβ(S177)抗体的3篇参考文献示例(注：文献为模拟示例，建议通过数据库验证实际引用)：
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1. **文献名称**：*"TAK1-dependent phosphorylation of IKKβ at Ser177 mediates NF-κB activation in response to TNF-α"*
**作者**：Karin M, Delhase M
**摘要**：研究证实TNF-α刺激下，TAK1激酶通过磷酸化IKKβ的S177位点激活IKK复合体，促进NF-κB信号通路，使用Phospho-IKKβ(S177)抗体验证了磷酸化动力学。
2. **文献名称**：*"Site-specific phosphorylation of IKKβ regulates inflammatory responses in macrophages"*
**作者**：Li Y, Chen ZJ
**摘要**：通过Phospho-IKKβ(S177)抗体分析LPS诱导的巨噬细胞，发现S177磷酸化是IKKβ激活的关键步骤，调控下游促炎因子分泌。
3. **文献名称**：*"Phospho-IKKβ(S177) as a biomarker for NF-κB activity in colorectal cancer"*
**作者**：Wang J, Gu H
**摘要**：利用Phospho-IKKβ(S177)抗体进行免疫组化，揭示该位点磷酸化水平与结直肠癌NF-κB持续活化及患者预后不良相关。
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**建议**：实际引用时可通过PubMed或Google Scholar搜索关键词“Phospho-IKKβ Ser177 antibody”或参考抗体厂商(如Cell Signaling Technology #2697)提供的文献列表获取准确信息。
Phospho-IKKβ (S177) antibodies are essential tools for studying the activation status of the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ), a key regulator of the NF-κB signaling pathway. IKKβ, part of the IKK complex (IKKα, IKKβ, and NEMO/IKKγ), phosphorylates the inhibitor of κB (IκB) proteins, leading to their degradation and subsequent activation of NF-κB transcription factors. Phosphorylation at serine 177 (and serine 181 in humans) within the activation loop of IKKβ is critical for its kinase activity, which is triggered by pro-inflammatory cytokines (e.g., TNF-α, IL-1β), pathogens, or stress signals. This post-translational modification is a hallmark of IKKβ activation, enabling downstream signaling in immune responses, inflammation, and cell survival.
Phospho-IKKβ (S177)-specific antibodies are designed to detect this phosphorylation event, allowing researchers to assess IKKβ activation in experimental models. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study signaling dynamics in diseases such as cancer, autoimmune disorders, and chronic inflammation. Validation of these antibodies typically involves verifying specificity using phosphorylation-deficient mutants or kinase inhibitors. Cross-reactivity with related kinases (e.g., IKKα) should be ruled out. By targeting S177 phosphorylation, these antibodies provide insights into NF-κB pathway regulation and potential therapeutic interventions targeting IKKβ activity.
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