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Rabbit Polyclonal Phospho-CCNB3(T280) Antibody

  • 中文名: Phospho-CCNB3(T280)抗体
  • 别    名: G2/mitotic-specific cyclin-B3, CCNB3, CYCB3
货号: IPDX32417
Price: ¥1280
数量:
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验证与应用

应用及物种
WB DB: 1/500 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesG2/mitotic-specific cyclin-B3, CCNB3, CYCB3
Entrez GeneID85417
WB Predicted band size157.9kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T280 of human CCNB3.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是基于Phospho-CCNB3(T280)抗体相关研究领域模拟的参考文献示例(注:文献为虚构,仅展示格式和内容结构):

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1. **"Phosphorylation of Cyclin B3 at Threonine 280 Regulates Meiotic Progression in Mouse Spermatocytes"**

*Authors: Zhang L, et al.*

**摘要**:本研究揭示了CCNB3在小鼠减数分裂中的磷酸化调控机制。通过制备T280位点特异性抗体,发现磷酸化修饰影响CCNB3与CDK1的结合,调控纺锤体组装检查点,为生殖细胞发育异常提供分子机制。

2. **"Development and Validation of a Phospho-Specific Antibody for Cyclin B3 (T280) in Human Cancers"**

*Authors: Müller R, et al.*

**摘要**:文章报道了一种针对CCNB3 T280磷酸化位点的单克隆抗体的开发,通过Western blot和免疫组化验证其特异性,并应用于乳腺癌样本分析,提示该位点磷酸化与肿瘤侵袭性相关。

3. **"T280 Phosphorylation Modulates Cyclin B3 Stability During the G2/M Transition"**

*Authors: Tanaka K, et al.*

**摘要**:利用Phospho-CCNB3(T280)抗体,研究发现CDK1介导的T280磷酸化通过泛素-蛋白酶体途径调控CCNB3降解,影响细胞周期进程和染色体分离。

4. **"A Functional Screen Identifies CCNB3 Phosphorylation as a Critical Regulator of Oocyte Maturation"**

*Authors: Chen Y, et al.*

**摘要**:通过Phospho-CCNB3(T280)抗体的应用,证实T280磷酸化在卵母细胞成熟中通过调控MPF(成熟促进因子)活性发挥作用,突变该位点导致减数分裂阻滞。

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**注**:以上文献为示例性内容,实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。若需具体论文,可提供更详细的研究背景或关键词进一步筛选。

背景信息

The Phospho-CCNB3(T280) antibody detects the phosphorylated form of Cyclin B3 (CCNB3) at threonine 280. a post-translational modification critical for regulating CCNB3 activity during the cell cycle. CCNB3. a member of the B-type cyclin family, plays a role in the transition from G2 to M phase by binding and activating cyclin-dependent kinase 1 (CDK1). Phosphorylation at T280 is associated with modulating CCNB3 stability, subcellular localization, or interaction with CDKs, though its precise regulatory mechanism remains under investigation. This antibody is particularly relevant in studies of cell cycle dynamics, checkpoint control, and diseases marked by cell cycle dysregulation, such as cancers. CCNB3 overexpression or dysregulation has been linked to certain malignancies, including a subset of sarcomas, where its aberrant activity may drive uncontrolled proliferation. Researchers use the Phospho-CCNB3(T280) antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation status, correlate it with cell cycle progression, or evaluate therapeutic responses in preclinical models. Its specificity for the phosphorylated epitope makes it a valuable tool for dissecting signaling pathways involving CCNB3 and exploring its role in oncogenesis or targeted therapies.

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