WB | DB: 1/500 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | G2/mitotic-specific cyclin-B3, CCNB3, CYCB3 |
Entrez GeneID | 85417 |
WB Predicted band size | 157.9kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T280 of human CCNB3. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是基于Phospho-CCNB3(T280)抗体相关研究领域模拟的参考文献示例(注:文献为虚构,仅展示格式和内容结构):
---
1. **"Phosphorylation of Cyclin B3 at Threonine 280 Regulates Meiotic Progression in Mouse Spermatocytes"**
*Authors: Zhang L, et al.*
**摘要**:本研究揭示了CCNB3在小鼠减数分裂中的磷酸化调控机制。通过制备T280位点特异性抗体,发现磷酸化修饰影响CCNB3与CDK1的结合,调控纺锤体组装检查点,为生殖细胞发育异常提供分子机制。
2. **"Development and Validation of a Phospho-Specific Antibody for Cyclin B3 (T280) in Human Cancers"**
*Authors: Müller R, et al.*
**摘要**:文章报道了一种针对CCNB3 T280磷酸化位点的单克隆抗体的开发,通过Western blot和免疫组化验证其特异性,并应用于乳腺癌样本分析,提示该位点磷酸化与肿瘤侵袭性相关。
3. **"T280 Phosphorylation Modulates Cyclin B3 Stability During the G2/M Transition"**
*Authors: Tanaka K, et al.*
**摘要**:利用Phospho-CCNB3(T280)抗体,研究发现CDK1介导的T280磷酸化通过泛素-蛋白酶体途径调控CCNB3降解,影响细胞周期进程和染色体分离。
4. **"A Functional Screen Identifies CCNB3 Phosphorylation as a Critical Regulator of Oocyte Maturation"**
*Authors: Chen Y, et al.*
**摘要**:通过Phospho-CCNB3(T280)抗体的应用,证实T280磷酸化在卵母细胞成熟中通过调控MPF(成熟促进因子)活性发挥作用,突变该位点导致减数分裂阻滞。
---
**注**:以上文献为示例性内容,实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。若需具体论文,可提供更详细的研究背景或关键词进一步筛选。
×