Rabbit Polyclonal Phospho-EP300(S1834) Antibody

The Phospho-EP300(S1834) antibody is designed to detect EP300 (E1A-binding protein p300), a ubiquitously expressed transcriptional coactivator, at its phosphorylated serine 1834 residue. EP300. along with its homolog CREBBP, plays a critical role in regulating gene expression by acetylating histones and non-histone proteins, modulating chromatin structure and transcriptional activity. The S1834 phosphorylation site resides within the EP300 histone acetyltransferase (HAT) domain, a region essential for its enzymatic activity. Phosphorylation at this site has been implicated in dynamically regulating EP300’s HAT function, potentially influencing its interaction with transcription factors (e.g., p53. HIF-1α) or its recruitment to chromatin. Studies suggest that S1834 phosphorylation may suppress EP300 activity, impacting downstream pathways involved in cell proliferation, differentiation, and stress responses. This antibody is widely used in basic and translational research to investigate phosphorylation-dependent regulatory mechanisms of EP300 in processes such as cancer progression, inflammation, and metabolic disorders. It is validated for applications including Western blotting, immunofluorescence, and immunoprecipitation, providing a tool to explore post-translational modifications that fine-tune EP300’s role in transcriptional networks and disease pathogenesis.