| WB | DB: 1/500 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Hamartin, Tuberous sclerosis 1 protein homolog, Tsc1 |
| Entrez GeneID | 60445 |
| WB Predicted band size | 129.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | This rat TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y297 of rat TSC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-rat TSC1(Y297)抗体的示例参考文献(注：由于该位点研究较为特异，以下为假设性文献，实际文献需通过学术数据库验证)：
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1. **"Phosphorylation of TSC1 at Y297 regulates mTORC1 signaling in response to growth factors"**
*Author: Li X et al.*
**摘要**：本研究揭示了大鼠TSC1蛋白Y297位点的磷酸化在生长因子激活mTORC1通路中的关键作用，通过Phospho-TSC1(Y297)抗体证实该修饰可抑制TSC1/TSC2复合体活性，促进Rheb-GTP依赖的mTORC1活化。
2. **"Tyrosine phosphorylation of TSC1 modulates cellular stress responses"**
*Author: Chen H et al.*
**摘要**：利用Phospho-TSC1(Y297)特异性抗体，作者发现氧化应激诱导的TSC1 Y297磷酸化通过调控mTORC1和自噬通路影响细胞存活，该修饰可能由Src家族激酶介导。
3. **"TSC1 phosphorylation at Y297 is required for tumor progression in a rat glioma model"**
*Author: Wang Y et al.*
**摘要**：在胶质瘤模型中，Phospho-TSC1(Y297)抗体的免疫组化分析显示，Y297磷酸化水平与肿瘤侵袭性正相关，提示其作为潜在治疗靶点的价值。
4. **"Development and validation of a phospho-specific antibody targeting rat TSC1 at tyrosine 297"**
*Author: Smith J et al.*
**摘要**：本文详细描述了Phospho-TSC1(Y297)抗体的制备与验证过程，包括其在Western blot和免疫荧光中的应用，确认其特异性及在能量敏感条件下的检测灵敏度。
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**提示**：实际研究中建议通过PubMed或Google Scholar以关键词“TSC1 Y297 phosphorylation”或抗体货号检索最新文献，亦可参考抗体供应商(如CST、Abcam)提供的产品引用文献。
The Phospho-rat TSC1 (Y297) antibody is a specialized tool for detecting the phosphorylation status of the tuberous sclerosis complex 1 (TSC1) protein at tyrosine residue 297 in rat-derived samples. TSC1. in complex with TSC2. acts as a critical regulator of the mTORC1 signaling pathway, which governs cell growth, proliferation, and metabolism. Phosphorylation events on TSC1/TSC2 modulate their activity, influencing their ability to inhibit mTORC1. The Y297 phosphorylation site in TSC1 is implicated in regulating the stability or function of the TSC complex, though its precise role remains under investigation. This antibody is particularly useful in studies exploring mTOR pathway dynamics in response to growth factors, cellular stress, or oncogenic signaling.
It is commonly employed in techniques like Western blotting or immunoprecipitation to assess TSC1 phosphorylation changes under experimental conditions such as growth factor stimulation, nutrient deprivation, or drug treatments. Researchers use it to investigate diseases linked to mTOR dysregulation, including cancer, neurological disorders, and metabolic syndromes. Specificity for the phosphorylated Y297 epitope is typically validated using knockout controls or phosphatase-treated samples.
Understanding TSC1 phosphorylation at Y297 may shed light on novel regulatory mechanisms of mTOR signaling, offering potential therapeutic targets for diseases driven by pathway hyperactivity. This antibody thus serves as a key reagent in both basic and translational research contexts.
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