| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C5a anaphylatoxin chemotactic receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5AR2, C5L2, GPR77 |
| Entrez GeneID | 27202 |
| WB Predicted band size | 36.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GPR77 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human GPR77. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GPR77抗体的3篇文献摘要信息(注：以下内容为模拟示例，具体文献需根据实际数据库检索)：
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1. **文献名称**: *GPR77 as a Biomarker for Cancer Stem Cells in Triple-Negative Breast Cancer*
**作者**: Li, X., et al.
**摘要**: 该研究利用GPR77抗体鉴定三阴性乳腺癌中的肿瘤干细胞群体，发现GPR77高表达与化疗抵抗和预后不良相关，提示其可作为潜在治疗靶点。
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2. **文献名称**: *GPR77 Regulates Neutrophil Recruitment in Acute Lung Injury*
**作者**: Chen, Y., et al.
**摘要**: 通过GPR77抗体阻断实验，研究揭示了GPR77在炎症微环境中调控中性粒细胞迁移的机制，为急性肺损伤的免疫治疗提供新思路。
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3. **文献名称**: *Development of a Monoclonal Antibody Against Human GPR77 for Flow Cytometry Applications*
**作者**: Smith, J., et al.
**摘要**: 本文报道了一种高特异性抗人GPR77单克隆抗体的开发，验证了其在流式细胞术中对免疫细胞表面GPR77的检测效能，适用于基础与临床研究。
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如需具体文献，建议通过PubMed或Web of Science搜索关键词“GPR77 antibody”获取最新研究。
GPR77. also known as C5a receptor 2 (C5aR2 or C5L2), is a member of the G protein-coupled receptor (GPCR) family that binds to complement component C5a, a potent inflammatory mediator involved in immune responses. Unlike its homolog C5aR1. GPR77 lacks classical G protein coupling and is thought to act as a decoy or modulatory receptor, fine-tuning C5a-mediated signaling. It plays dual roles in inflammation, exhibiting both pro- and anti-inflammatory effects depending on cellular context. GPR77 is expressed in immune cells (e.g., neutrophils, macrophages), endothelial cells, and certain cancer cells, linking it to diseases like sepsis, rheumatoid arthritis, and tumor progression.
Antibodies targeting GPR77 are critical tools for studying its expression, localization, and functional mechanisms. They enable detection via techniques such as Western blotting, immunofluorescence, and flow cytometry, helping to map receptor distribution in tissues and disease models. Recent research highlights GPR77's role in maintaining cancer stem cell survival in tumors like breast cancer, making its antibodies valuable for therapeutic exploration. However, challenges remain in distinguishing GPR77 from homologous receptors due to structural similarities. Validated antibodies with high specificity are essential for clarifying its controversial signaling pathways and advancing drug development aimed at modulating C5a-related inflammatory diseases or cancer microenvironments.
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