| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B, HsPDE8B, Cell proliferation-inducing gene 22 protein, PDE8B |
| Entrez GeneID | 8622 |
| WB Predicted band size | 99.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This PDE8B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-324 amino acids from the Central region of human PDE8B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"Phosphodiesterase 8B (PDE8B) expression in thyroid neoplasms: a tissue microarray study"**
- Author: J. Smith et al.
- 摘要：研究通过免疫组化分析PDE8B抗体在甲状腺肿瘤组织中的表达，发现PDE8B在滤泡性癌中高表达，提示其作为潜在诊断标志物的可能性。
2. **"Characterization of PDE8B-specific antibodies for detecting cAMP signaling dynamics in ovarian cancer"**
- Author: L. Chen & R. Kumar
- 摘要：开发并验证了高特异性PDE8B抗体，用于Western blot和免疫荧光，揭示了PDE8B在卵巢癌细胞中调控cAMP-PKA通路的机制。
3. **"PDE8B regulates cortisol metabolism in adrenal insufficiency: an antibody-based proteomic approach"**
- Author: M. Tanaka et al.
- 摘要：利用PDE8B抗体进行蛋白质组学分析，证明PDE8B通过调控皮质醇代谢酶活性参与肾上腺功能不全的病理过程。
4. **"Localization of PDE8B in the murine brain using a novel monoclonal antibody"**
- Author: K. Patel et al.
- 摘要：报道了一种新型PDE8B单克隆抗体的开发，并通过免疫组织化学证实其在小鼠脑组织特定区域(如海马和皮层)的高表达。
(注：上述文献为示例，实际引用时需根据真实文献调整。)
PDE8B (phosphodiesterase 8B) is a member of the phosphodiesterase enzyme family, which regulates cyclic nucleotide signaling by hydrolyzing cAMP and cGMP. Specifically, PDE8B preferentially degrades cAMP, playing a critical role in modulating cAMP-dependent pathways involved in cellular processes like metabolism, inflammation, and hormone secretion. This enzyme is highly expressed in tissues such as the thyroid, adrenal glands, brain, and testes, where cAMP signaling is tightly regulated.
Antibodies targeting PDE8B are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to detect PDE8B protein levels in research models or clinical samples. Dysregulation of PDE8B has been implicated in endocrine disorders, including thyroid dysfunctions and adrenal hyperplasia, as well as neurological conditions. For instance, PDE8B mutations or altered expression may disrupt steroidogenesis or cAMP-mediated neuronal signaling.
Commercial PDE8B antibodies are typically developed using immunogenic peptides or recombinant proteins, with validation in specific applications and species. Researchers prioritize antibodies with high specificity due to structural similarities among PDE family members. Studying PDE8B also contributes to drug discovery, as PDE inhibitors are explored for treating diseases linked to cAMP signaling imbalances. Overall, PDE8B antibodies are vital for unraveling its role in health and disease.
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