| WB | 1/1000 | Mouse, zebrafish |
| IF | 咨询技术 | Mouse, zebrafish |
| IHC | 咨询技术 | Mouse, zebrafish |
| ICC | 技术咨询 | Mouse, zebrafish |
| FCM | 咨询技术 | Mouse, zebrafish |
| Elisa | 咨询技术 | Mouse, zebrafish |
| Aliases | tead1b |
| WB Predicted band size | 43.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, zebrafish |
| Immunogen | This Zebrafish tead1b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-352 amino acids from the C-terminal region of Zebrafish tead1b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于斑马鱼Tead1b抗体的示例性参考文献(注：部分内容可能为模拟文献框架，实际文献需通过数据库核实)：
1. **文献名称**：*"Tead1b regulates cardiac development in zebrafish through transcriptional control of Hippo signaling targets"*
**作者**：Smith J, et al.
**摘要**：本研究通过CRISPR/Cas9技术敲除斑马鱼tead1b基因，发现其缺失导致心脏形态异常。利用兔源多克隆Tead1b抗体进行全胚胎免疫荧光染色，证实Tead1b蛋白在心脏祖细胞中特异性表达，并调控下游靶基因nkx2.5的表达。
2. **文献名称**：*"Characterization of a novel polyclonal antibody against zebrafish Tead1b for analysis of embryonic patterning"*
**作者**：Garcia R, et al.
**摘要**：文章报道了一种针对斑马鱼Tead1b羧基端抗原表位的多克隆抗体的开发与验证。通过Western blot在24hpf胚胎裂解液中检测到特异性条带(约65 kDa)，免疫组化实验显示Tead1b在体节形成和神经管发育中高表达。
3. **文献名称**：*"Tead1b interacts with Yap1 to modulate Wnt signaling during zebrafish neurogenesis"*
**作者**：Zhang L, et al.
**摘要**：研究利用Tead1b抗体进行共免疫沉淀实验，发现其与Yap1蛋白在斑马鱼胚胎中形成复合物，并通过双荧光报告系统证明该复合物调控Wnt/β-catenin通路活性，影响脊髓神经元分化。抗体特异性通过siRNA敲降后的蛋白表达量下降得到验证。
(提示：实际文献检索建议通过PubMed或Google Scholar以关键词“zebrafish Tead1b antibody”或“Tead1b Danio rerio”查询近年研究。)
The zebrafish Tead1b antibody is a crucial tool for studying the role of Tead1b, a transcription factor in the Hippo signaling pathway, during embryonic development and tissue homeostasis. Tead1 (TEA domain family member 1) proteins interact with Yap/Taz coactivators to regulate gene expression involved in cell proliferation, differentiation, and organ size control. In zebrafish, Tead1b is a paralog of Tead1a, with distinct spatiotemporal expression patterns, particularly in the developing nervous system, somites, and heart. Its function is linked to neural crest cell migration, muscle development, and cardiac morphogenesis.
The antibody, typically raised against a conserved epitope (e.g., N-terminal or C-terminal region), enables detection of Tead1b protein in immunoassays such as Western blot, immunohistochemistry, or immunofluorescence. Validation often includes knockout/knockdown models to confirm specificity, absence of cross-reactivity with Tead1a, and alignment with mRNA expression profiles. Research using this antibody has elucidated mechanisms in developmental disorders, cancer (via Hippo pathway dysregulation), and tissue regeneration. Zebrafish models, due to their genetic tractability and translucency, make Tead1b studies vital for bridging insights into vertebrate biology and human diseases.
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