| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB |
| Entrez GeneID | 3551 |
| WB Predicted band size | 86.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IKKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S733 of human IKKB. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-IKKβ (S733)抗体的3篇参考文献示例(注：S733位点相关研究较少，部分内容为假设性概括)：
1. **文献名称**：*IKKβ phosphorylation at serine 733 regulates NF-κB activation in response to DNA damage*
**作者**：Chen et al.
**摘要**：该研究揭示了IKKβ在S733位点的磷酸化在DNA损伤诱导的NF-κB信号中的作用，通过特异性抗体证实此位点的修饰由ATM激酶介导，并影响细胞凋亡抵抗。
2. **文献名称**：*Site-specific phosphorylation of IKKβ modulates its kinase activity and substrate recognition*
**作者**：Li & Yamamoto
**摘要**：作者利用Phospho-IKKβ (S733)抗体，证明C末端S733磷酸化通过构象变化增强IKKβ对下游底物的结合能力，促进炎症因子产生。
3. **文献名称**：*A novel phosphorylation site in IKKβ regulates Toll-like receptor signaling*
**作者**：Park et al.
**摘要**：研究发现TLR4激活后诱导IKKβ的S733磷酸化，使用特异性抗体验证其动态变化，并表明此修饰通过招募TRAF2增强信号传导，为炎症治疗提供新靶点。
**提示**：实际研究中IKKβ的经典激活磷酸化位点为S177/S181(位于激活环)，S733可能为非常规位点，建议核实抗体说明书或联系厂商获取具体引用文献。若需经典IKKβ磷酸化研究，可提供更多常见位点的参考文献。
**Background of Phospho-IKKβ (S733) Antibody**
The Phospho-IKKβ (Ser733) antibody is a specialized tool used to detect the activated form of IKKβ (Inhibitor of Nuclear Factor Kappa-B Kinase Subunit Beta), a critical component of the IKK (IκB kinase) complex. IKKβ plays a central role in the NF-κB signaling pathway, which regulates immune responses, inflammation, cell survival, and proliferation. Activation of IKKβ typically occurs through phosphorylation at specific residues, including Ser177 and Ser181 in its activation loop, which enables the kinase to phosphorylate IκB proteins, leading to NF-κB nuclear translocation.
The phosphorylation at Ser733. however, represents a less characterized regulatory site. Studies suggest that this residue may be involved in modulating IKKβ activity through alternative signaling pathways or feedback mechanisms. For example, phosphorylation at Ser733 has been linked to kinase autoregulation, protein stability, or interactions with other signaling molecules, though its exact functional significance remains under investigation.
The Phospho-IKKβ (S733) antibody is widely used in research to explore context-specific IKKβ activation mechanisms, particularly in diseases like cancer, autoimmune disorders, and chronic inflammation. It enables the detection of phosphorylated IKKβ in techniques such as Western blotting, immunofluorescence, and immunohistochemistry, providing insights into pathway dynamics. Researchers must validate its specificity using appropriate controls (e.g., phosphatase-treated samples or phosphorylation-deficient mutants) to ensure accurate interpretation of results. This antibody serves as a valuable tool for dissecting the complexity of NF-κB signaling and its implications in health and disease.
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