Rabbit Polyclonal MKRN1(Center) Antibody

The MKRN1 (Center) antibody is a commonly used tool for detecting the middle region of the human makorin ring finger protein 1 (MKRN1), a ubiquitously expressed E3 ubiquitin ligase involved in protein degradation via the ubiquitin-proteasome system. MKRN1 belongs to the makorin family, characterized by a unique combination of zinc-finger motifs and a RING finger domain critical for its enzymatic activity. It regulates diverse cellular processes, including cell proliferation, apoptosis, and immune responses, and has been implicated in neurodegenerative diseases, cancer, and viral infection pathways. The "Center" designation refers to the antibody's epitope targeting the central portion of MKRN1 (often around amino acids 200-300), avoiding conserved domains to enhance specificity. This polyclonal or monoclonal antibody is typically raised in hosts like rabbits or mice, validated in applications such as Western blotting, immunoprecipitation, and immunofluorescence. Its reliability is often confirmed using MKRN1 knockout cell lines as negative controls. Researchers employ this antibody to study MKRN1's role in modulating substrates like p53. telomerase, or viral proteins, as well as its tissue-specific expression patterns. Its development has advanced understanding of MKRN1's dual roles as both an oncogene and tumor suppressor, depending on cellular context.