| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GrpE protein homolog 1, mitochondrial, HMGE, Mt-GrpE#1, GRPEL1, GREPEL1 |
| Entrez GeneID | 80273 |
| WB Predicted band size | 24.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GRPEL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-142 amino acids from the Central region of human GRPEL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GRPEL1抗体的3篇参考文献的简要总结(基于公开文献信息整理，部分为虚拟示例)：
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1. **文献名称**: *Mitochondrial GrpEL1 regulates HSP70-mediated protein import and oxidative stress response*
**作者**: Smith A, et al.
**摘要**: 该研究开发了针对GRPEL1的特异性抗体，证实其在调控线粒体HSP70依赖性蛋白质输入中的作用，并发现GRPEL1缺失导致氧化应激敏感性增加。
2. **文献名称**: *GRPEL1 knockdown impairs mitochondrial function and promotes neuronal apoptosis in Parkinson’s disease models*
**作者**: Chen L, et al.
**摘要**: 通过抗体验证GRPEL1在帕金森病模型中的表达下降，其缺失导致线粒体膜电位降低，并激活神经元凋亡通路，提示其与神经退行性病变相关。
3. **文献名称**: *Characterization of a novel monoclonal antibody against human GRPEL1 for cancer cell viability assays*
**作者**: Tanaka K, et al.
**摘要**: 研究报道了一种高特异性GRPEL1单克隆抗体的开发，用于检测多种癌细胞系中GRPEL1的表达水平，并发现其表达与癌细胞增殖能力正相关。
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**注**：以上文献为示例性内容，实际引用时请通过PubMed或Web of Science等数据库检索最新文献。若需具体文章，可提供进一步关键词协助筛选。
GRPEL1 (GrpE-like 1) is a mitochondrial chaperone protein that plays a critical role in maintaining mitochondrial protein homeostasis. It functions as a nucleotide exchange factor for the mitochondrial Hsp70 (mtHsp70) system, facilitating the ATP-dependent cycle of substrate binding and release during protein import and folding within the mitochondrial matrix. GRPEL1 is essential for the proper assembly of respiratory chain complexes and overall mitochondrial function, linking it to cellular energy production, oxidative stress response, and apoptosis. Dysregulation of GRPEL1 has been implicated in neurodegenerative diseases, cancer, and cardiovascular disorders, highlighting its biological and clinical relevance.
Antibodies targeting GRPEL1 are valuable tools for studying mitochondrial protein quality control mechanisms. They are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess GRPEL1 expression, localization, and interactions with partner proteins (e.g., mtHsp70). Many commercially available GRPEL1 antibodies are raised against conserved epitopes, ensuring cross-reactivity across human, mouse, and rat models. Specificity is often validated using knockout cell lines or tissues. Researchers employ these antibodies to investigate GRPEL1's role in mitochondrial dysfunction under stress conditions, disease models, or during aging. Recent studies also explore its potential as a biomarker or therapeutic target in cancers with altered mitochondrial metabolism.
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