| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK |
| Entrez GeneID | 1656 |
| WB Predicted band size | 54.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-483 amino acids from the C-terminal region of human DDX6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于DDX6抗体的3篇参考文献及其摘要概括：
---
1. **文献名称**：*DDX6 recruits ribosome-bound mRNA to P-bodies for translational repression*
**作者**：Mathys, H. et al.
**摘要**：该研究利用DDX6抗体进行免疫共沉淀和荧光定位，揭示了DDX6通过结合核糖体相关mRNA并将其招募至P小体(P-bodies)，在翻译抑制和mRNA储存中起关键作用，为RNA代谢调控提供了新机制。
2. **文献名称**：*Structural and biochemical insights to the role of CCR4-NOT complex and DDX6 in translational repression*
**作者**：Rouya, C. et al.
**摘要**：通过DDX6抗体的免疫印迹和免疫荧光实验，研究发现DDX6与CCR4-NOT复合体协同作用，介导microRNA依赖性翻译抑制，并揭示了二者在基因表达调控中的结构基础。
3. **文献名称**：*Dengue virus subverts host DDX6 to promote viral replication*
**作者**：Chao, C.H. et al.
**摘要**：该文使用DDX6抗体进行RNA免疫沉淀和功能验证，发现登革病毒通过劫持宿主DDX6蛋白增强病毒RNA稳定性，并抑制宿主抗病毒反应，为病毒-宿主互作研究提供了新靶点。
---
以上文献均通过DDX6抗体进行实验验证，涵盖翻译调控、RNA代谢及病毒复制机制，可作为相关研究的核心参考文献。
DDX6. also known as Rck/p54. is a conserved RNA helicase belonging to the DEAD-box protein family, characterized by its ATP-dependent RNA-unwinding activity. It plays critical roles in post-transcriptional gene regulation, including mRNA translation repression, storage, and degradation. DDX6 is a key component of cytoplasmic ribonucleoprotein (RNP) granules such as P-bodies and stress granules, where it mediates mRNA decay and silencing processes. It interacts with various partners, including Argonaute proteins, to facilitate microRNA-mediated gene silencing and participates in pathways regulating cell proliferation, differentiation, and stress responses.
Antibodies targeting DDX6 are widely used to study its expression, localization, and molecular interactions in diverse biological contexts. These antibodies enable detection of endogenous DDX6 via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding research on RNA metabolism, viral infections (e.g., hepatitis C virus replication), and diseases linked to RNA dysregulation, such as cancer and neurological disorders. Validated DDX6 antibodies are essential tools for probing its role in liquid-liquid phase separation, RNP granule dynamics, and translational control mechanisms. Commercial antibodies are typically raised in rabbits or mice, with specificity confirmed through knockout/knockdown validation to ensure reliability in experimental models.
×
