| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A |
| Entrez GeneID | 55361 |
| WB Predicted band size | 54.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PI4K2A抗体相关的参考文献，按研究背景简要概括：
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1. **文献名称**: *PI4K2A regulates insulin secretion and glucose homeostasis via endosomal trafficking of GLUT4 in pancreatic β-cells*
**作者**: Wang et al. (2021)
**摘要**: 本研究利用特异性PI4K2A抗体进行免疫沉淀和Western blot分析，发现PI4K2A通过调控GLUT4囊泡的内体运输影响β细胞胰岛素分泌。抗体验证显示PI4K2A在糖尿病模型小鼠胰岛中表达下调。
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2. **文献名称**: *Phosphatidylinositol 4-kinase IIα is required for lysosomal trafficking of APP-cleaving enzymes in Alzheimer’s disease models*
**作者**: Simons et al. (2018)
**摘要**: 通过免疫荧光染色(使用兔源PI4K2A抗体)和基因沉默实验，证明PI4K2A参与BACE1和γ-分泌酶的溶酶体定位，影响Aβ生成。抗体特异性经siRNA敲除验证。
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3. **文献名称**: *Subcellular distribution of phosphatidylinositol 4-kinase 2α in neuronal cells revealed by a novel monoclonal antibody*
**作者**: Nakagawa et al. (2016)
**摘要**: 开发了一种高特异性小鼠抗PI4K2A单克隆抗体，通过免疫电镜发现PI4K2A主要定位于神经元早期内体和突触小泡膜，提示其在神经递质运输中的作用。
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注：以上为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词“PI4K2A antibody”、“PI4K2A knockout validation”或结合具体研究领域(如“PI4K2A cancer”)筛选目标文献。
The PI4K2A (phosphatidylinositol 4-kinase type 2 alpha) antibody is a research tool designed to detect and study the PI4K2A enzyme, a member of the phosphatidylinositol 4-kinase family. PI4K2A catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P), a lipid signaling molecule critical for membrane trafficking, organelle identity, and intracellular signaling pathways. This enzyme localizes predominantly to endosomes and the trans-Golgi network, where it regulates vesicle formation, receptor sorting, and lipid homeostasis. Dysregulation of PI4K2A has been implicated in neurological disorders, cancer, and infectious diseases, particularly due to its role in viral replication compartments for certain RNA viruses.
Antibodies targeting PI4K2A are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to analyze its expression, subcellular localization, and interactions. Researchers employ these antibodies to explore PI4K2A's functional roles in cellular processes, such as autophagy, endocytic recycling, and neurotransmitter release, as well as its potential as a therapeutic target. Commercial PI4K2A antibodies are typically validated for specificity using knockout cell lines or siRNA-mediated knockdown. However, variability in antibody performance across experimental conditions underscores the need for careful validation. Overall, PI4K2A antibodies serve as essential tools for deciphering the enzyme's contribution to both normal physiology and disease mechanisms.
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