| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Translation initiation factor IF-2, mitochondrial, IF-2(Mt), IF-2Mt, IF2(mt), MTIF2 |
| Entrez GeneID | 4528 |
| WB Predicted band size | 81.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MTIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human MTIF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MTIF2(N-term)抗体的3篇参考文献的示例(注：以下为模拟内容，实际文献需通过学术数据库验证)：
1. **《Mitochondrial translation initiation factor 2 (MTIF2) antibody validation for functional studies》**
- 作者：Smith, J. et al.
- 摘要：该研究报道了一种针对MTIF2蛋白N端区域的多克隆抗体的开发与验证，通过Western blot和免疫荧光实验证实其特异性，并用于探究MTIF2在线粒体翻译起始中的作用。
2. **《MTIF2 expression dynamics in human cardiomyopathies》**
- 作者：Lee, H. & Chen, R.
- 摘要：文章利用MTIF2(N-term)抗体分析心肌病患者组织中MTIF2的蛋白水平，发现其表达与线粒体功能异常相关，提示其作为疾病生物标志物的潜力。
3. **《Subcellular localization of MTIF2 in mammalian cells using N-terminal specific antibodies》**
- 作者：Garcia, M. et al.
- 摘要：通过MTIF2(N-term)抗体的免疫细胞化学实验，揭示了MTIF2在线粒体内膜附近的定位，并探讨其与核糖体组装的关联。
如需真实文献，建议通过PubMed或Google Scholar搜索关键词“MTIF2 antibody”或“MTIF2 N-terminal”进行查阅。
The MTIF2 (N-term) antibody is designed to target the N-terminal region of mitochondrial translation initiation factor 2 (MTIF2), a critical protein involved in mitochondrial protein synthesis. MTIF2 plays an essential role in initiating translation within mitochondria by facilitating the assembly of the mitochondrial ribosome and recruiting initiator tRNA to the small ribosomal subunit. This process is vital for synthesizing mitochondrial DNA-encoded proteins, particularly subunits of the electron transport chain complexes, which are crucial for cellular energy production.
The antibody is commonly used in research to study mitochondrial gene expression, translational regulation, and mitochondrial dysfunction linked to diseases such as metabolic disorders, neurodegenerative conditions, and cancer. By detecting MTIF2 via techniques like Western blotting, immunofluorescence, or immunoprecipitation, researchers can assess its expression levels, subcellular localization, and interactions with other mitochondrial components.
MTIF2 (N-term) antibodies are often validated for specificity using knockout or knockdown models to ensure minimal cross-reactivity with unrelated proteins. Their application extends to both diagnostic and mechanistic studies, helping elucidate how mitochondrial translation defects contribute to pathological states. This tool is indispensable for advancing understanding of mitochondrial biology and its implications in health and disease.
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