| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GPN-loop GTPase 1, 365-, MBD2-interacting protein, MBDin, RNAPII-associated protein 4, XPA-binding protein 1, GPN1 {ECO:0000303|PubMed:20855544, ECO:0000312|HGNC:HGNC:17030} |
| Entrez GeneID | 11321 |
| WB Predicted band size | 41.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-352 amino acids from the C-terminal region of human GPN1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GPN1抗体的假设性参考文献示例(实际文献可能需要进一步验证)：
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1. **文献名称**：*Structural and functional analysis of the GPN-loop GTPase GPN1 in RNA polymerase II assembly*
**作者**：Azouaoui, S., et al.
**摘要**：该研究通过X射线晶体学解析了GPN1的蛋白结构，并利用GPN1抗体验证其在RNA聚合酶II核质运输中的关键作用，发现GPN1突变会破坏RNA聚合酶的组装。
2. **文献名称**：*GPN1 overexpression promotes tumorigenesis in breast cancer via regulating cell cycle progression*
**作者**：Liu, Y., et al.
**摘要**：通过免疫组化(使用GPN1抗体)发现GPN1在乳腺癌组织中高表达，实验表明其通过调控细胞周期蛋白加速肿瘤增殖，提示GPN1作为潜在治疗靶点。
3. **文献名称**：*GPN1 interacts with the 26S proteasome and modulates protein degradation pathways*
**作者**：Rosado-Ivars, S., et al.
**摘要**：研究利用免疫共沉淀(GPN1抗体)证明GPN1与蛋白酶体亚基结合，影响错误折叠蛋白的清除，揭示其在蛋白质稳态中的新功能。
4. **文献名称**：*Antibody-based inhibition of GPN1 GTPase activity suppresses viral replication in vitro*
**作者**：Zhang, R., et al.
**摘要**：开发了特异性靶向GPN1的抗体，体外实验显示其可通过抑制GPN1的GTP酶活性阻断某些RNA病毒的复制机制。
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**注意**：以上文献为示例性质，具体内容需根据实际研究调整。建议通过PubMed或Google Scholar以关键词“GPN1 antibody”或“GPN1 function”检索最新文献。
The GPN1 antibody targets GPN-loop GTPase 1 (GPN1), a conserved eukaryotic protein critical for RNA polymerase II (RNAPII) assembly and nuclear transport. GPN1. part of the GPN-loop GTPase family, facilitates RNAPII subunit RPB1 translocation from the cytoplasm to the nucleus, ensuring proper transcriptional machinery function. It also interacts with the endoplasmic reticulum (ER) and Golgi apparatus, suggesting roles in protein trafficking. Dysregulation of GPN1 is linked to transcriptional defects, genomic instability, and diseases like cancer or neurodegeneration.
Antibodies against GPN1 are essential tools for studying its expression, localization, and molecular interactions. They enable applications such as Western blotting, immunofluorescence, and co-immunoprecipitation, helping elucidate GPN1's role in RNAPII biogenesis, DNA repair, and cell cycle regulation. Recent studies highlight GPN1's potential as a biomarker or therapeutic target in malignancies, given its overexpression in certain tumors.
Developed in hosts like rabbits or mice, GPN1 antibodies are validated for specificity and reproducibility, supporting both basic research and clinical investigations into transcription-related pathologies. Their use advances understanding of how GTPase-mediated processes impact cellular homeostasis and disease mechanisms.
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