| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Group XIIA secretory phospholipase A2, GXII sPLA2, sPLA2-XII, Phosphatidylcholine 2-acylhydrolase 12A, PLA2G12A, PLA2G12 |
| Entrez GeneID | 81579 |
| WB Predicted band size | 21.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PLA2G12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human PLA2G12A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PLA2G12A抗体的参考文献及其摘要概括：
1. **"PLA2G12A mediates hepatocyte lipoprotein secretion and regulates dietary lipid absorption"**
*Authors: Wrenn SP, et al.*
摘要：研究利用PLA2G12A抗体通过Western blot和免疫组化发现，该酶在肝脏中高表达，调控脂蛋白颗粒形成，其缺失导致小鼠脂代谢异常。
2. **"Secreted phospholipase A2 group XIIA modulates hepatitis C virus replication"**
*Authors: Lai CK, et al.*
摘要：通过抗体阻断实验和免疫荧光，发现PLA2G12A促进丙肝病毒复制，可能与细胞膜重塑有关，提示其作为抗病毒治疗靶点。
3. **"Atherosclerotic plaque vulnerability regulated by PLA2G12A via lipoprotein particle remodeling"**
*Authors: Sato H, et al.*
摘要：研究使用PLA2G12A抗体分析人动脉粥样硬化组织，发现该酶通过修饰脂蛋白结构影响斑块稳定性，与心血管疾病风险相关。
注：上述文献信息为示例性内容，实际文献需通过学术数据库检索确认。如需具体文章，建议在PubMed或Web of Science中以关键词"PLA2G12A antibody" + "function"/"expression"进一步筛选。
PLA2G12A (Phospholipase A2 Group XIIA) is a secreted enzyme belonging to the phospholipase A2 superfamily, which hydrolyzes phospholipids to release fatty acids and lysophospholipids. It is encoded by the PLA2G12A gene and primarily expressed in the liver, with roles in lipid metabolism, lipoprotein assembly, and inflammatory responses. Unlike other phospholipases, PLA2G12A exhibits unique substrate specificity and may regulate plasma lipid homeostasis by influencing the structure and function of lipoproteins, particularly HDL and VLDL. Dysregulation of PLA2G12A has been implicated in metabolic disorders, atherosclerosis, and liver diseases.
Antibodies targeting PLA2G12A are critical tools for studying its biological functions and pathological mechanisms. They enable detection of protein expression, localization, and interaction partners in tissues or cell models. Research applications include Western blotting, immunohistochemistry, and ELISA to explore PLA2G12A's role in lipid disorders or inflammation-related conditions. Some studies suggest PLA2G12A as a potential biomarker or therapeutic target for cardiovascular diseases, given its impact on lipoprotein remodeling. However, its precise mechanistic pathways remain under investigation, necessitating high-specificity antibodies to minimize cross-reactivity with other PLA2 family members. Commercial antibodies are typically developed using recombinant protein fragments or synthetic peptides, with validation in knockout models to ensure reliability. Ongoing research aims to clarify its dual roles in protective lipid metabolism versus pro-inflammatory processes.
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