| WB | 1/1000-1/8000 | Rat |
| IF | 咨询技术 | Rat |
| IHC | 咨询技术 | Rat |
| ICC | 技术咨询 | Rat |
| FCM | 咨询技术 | Rat |
| Elisa | 咨询技术 | Rat |
| Aliases | Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, Camk2a |
| Entrez GeneID | 25400 |
| WB Predicted band size | 54.1kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Rat |
| Immunogen | Purified His-tagged Camk2a protein(Fragment) was used to produced this monoclonal antibody. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于大鼠Camk2a抗体的虚拟参考文献示例(非真实文献，仅供格式参考)：
1. **文献名称**: "Specificity validation of anti-Camk2a antibodies in rat hippocampal neurons"
**作者**: Thompson, L. et al.
**摘要**: 本研究系统评估了三种商业来源Camk2a抗体在大鼠海马组织中的特异性。通过Western blot和免疫荧光验证，抗体#ABX-204在敲除模型中表现出最高特异性，适用于突触蛋白定位研究。
2. **文献名称**: "Camk2a phosphorylation dynamics in learning-associated rat prefrontal cortex"
**作者**: Suzuki, M. & Yamamoto, K.
**摘要**: 利用Camk2a单克隆抗体(克隆号6G9)，作者揭示了大鼠前额叶皮层在恐惧条件反射训练后Camk2a磷酸化水平的时空变化，提示其与记忆巩固的关联。
3. **文献名称**: "Comparative analysis of Camk2a isoform antibodies in rodent stroke models"
**作者**: Rodriguez, S. et al.
**摘要**: 对比研究显示，抗体#sc-130401能特异性识别大鼠脑缺血模型中Camk2aα亚型，在免疫组化中清晰显示皮层神经元活性变化，为中风研究提供可靠工具。
4. **文献名称**: "Antibody-based profiling of Camk2a expression in rat neurodegenerative models"
**作者**: Gupta, R. & Lee, T.
**摘要**: 通过优化Camk2a多克隆抗体(批号AC-2020)的抗原修复方法，成功应用于帕金森病大鼠模型的纹状体切片染色，揭示疾病进展中Camk2a表达下调现象。
注：以上为模拟文献，实际引用时需查询真实数据库(PubMed/Google Scholar)并核实详细信息。
The rat Camk2a antibody is a widely used tool in neuroscience research targeting the calcium/calmodulin-dependent protein kinase II alpha (Camk2a), a key enzyme in synaptic plasticity and memory formation. Camk2a, highly expressed in neurons, regulates calcium signaling pathways, influencing synaptic strength, gene expression, and long-term potentiation (LTP). The antibody specifically recognizes rat Camk2a, enabling applications like immunohistochemistry, Western blotting, and immunofluorescence to study protein localization, expression levels, and activity-dependent changes in rodent brain tissues.
Developed through immunization with purified or recombinant Camk2a fragments, these antibodies are often validated for specificity using knockout controls or siRNA knockdown. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, affecting experimental versatility. Researchers rely on Camk2a antibodies to investigate neurological disorders, such as Alzheimer’s disease, epilepsy, and schizophrenia, where Camk2a dysregulation is implicated. Its role in excitotoxicity and synaptic dysfunction also makes it relevant for stroke and trauma studies. Proper controls, including tissue from Camk2a-deficient models, are critical to confirm signal specificity, ensuring accurate interpretation in complex neural circuits.
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