| WB | 1/4000-1/64000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2, GBX2 |
| Entrez GeneID | 2637 |
| WB Predicted band size | 37.3kDa |
| Host/Isotype | Mouse IgM |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GBX2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the Central region of human GBX2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GBX2抗体与腹水(Ascites)研究的示例性参考文献(注：部分文献为假设性概括，实际需通过学术数据库验证)：
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1. **文献名称**：*GBX2 Expression in Ovarian Cancer Ascites and Its Role in Tumor Invasion*
**作者**：Smith A, et al.
**摘要**：本研究探讨了转录因子GBX2在卵巢癌腹水中的表达水平，发现其高表达与肿瘤细胞的侵袭性增强相关。通过免疫组化(使用抗GBX2抗体)证实，GBX2可能通过调控EMT通路促进癌细胞扩散至腹腔积液。
2. **文献名称**：*Development of a Novel Anti-GBX2 Monoclonal Antibody for Ascites-Based Diagnostics*
**作者**：Li Y, et al.
**摘要**：研究团队开发了一种特异性抗GBX2单克隆抗体，并验证其在肝癌腹水样本中的诊断潜力。实验表明，该抗体能高效识别腹水中的GBX2阳性循环肿瘤细胞，为液体活检提供新工具。
3. **文献名称**：*GBX2 as a Prognostic Marker in Malignant Ascites: Insights from Clinical Cohorts*
**作者**：Wang X, et al.
**摘要**：通过分析胃癌患者的腹水样本，研究发现GBX2蛋白水平(通过Western blot和抗体检测)与患者生存率显著相关，提示GBX2可作为恶性腹水的预后生物标志物。
4. **文献名称**：*Ascites-Derived GBX2 Promotes Angiogenesis in Colorectal Cancer Metastasis*
**作者**：Garcia R, et al.
**摘要**：该研究利用抗GBX2抗体阻断实验，证明腹水微环境中的GBX2通过激活VEGF通路促进血管生成，从而加速结直肠癌腹腔转移。
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**提示**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索最新文献，并注意结合具体研究背景选择参考资料。
The GBX2 (Ascites) antibody is a monoclonal antibody targeting the Gastrulation Brain Homeobox 2 (GBX2) protein, a member of the homeobox transcription factor family. GBX2 plays critical roles in embryonic development, particularly in neural patterning, hindbrain segmentation, and body axis formation. It regulates gene expression during morphogenesis by binding to specific DNA sequences, influencing cell differentiation and tissue organization. Dysregulation of GBX2 has been implicated in developmental disorders and cancers, making it a focus of research in developmental biology and oncology.
The "Ascites" designation indicates that the antibody was produced using the ascites method, where hybridoma cells (antibody-secreting cells fused with myeloma cells) are injected into the peritoneal cavity of mice or rats. This triggers fluid accumulation (ascites) rich in antibodies, which are then harvested and purified. While ascites production yields high antibody concentrations, ethical concerns over animal welfare have led to increased use of in vitro culture systems as alternatives.
The GBX2 (Ascites) antibody is widely used in techniques like immunohistochemistry (IHC), Western blotting, and immunofluorescence to study GBX2 expression, localization, and function in tissues or cell lines. Researchers employ it to explore GBX2's role in developmental pathways, cancer progression, or regenerative processes. However, users must validate its specificity via controls, as cross-reactivity with related proteins (e.g., other homeobox factors) may occur. Commercial suppliers often provide detailed protocols and validation data to ensure reproducibility.
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