| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cullin-1, CUL-1, CUL1 |
| Entrez GeneID | 8454 |
| WB Predicted band size | 89.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CUL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 680-708 amino acids from the C-terminal region of human CUL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CUL1抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*"CUL1 regulates cell cycle progression through ubiquitination of cyclin-dependent kinase inhibitors"*
**作者**：Singer JD et al.
**摘要**：研究通过CUL1抗体进行免疫沉淀和Western blot分析，揭示了CUL1在SCF复合体中的作用，其通过泛素化降解细胞周期蛋白依赖性激酶抑制剂(如p27)，调控细胞周期进程。
2. **文献名称**：*"CUL1 expression and its clinical significance in colorectal cancer"*
**作者**：Wang L et al.
**摘要**：利用CUL1抗体进行免疫组织化学染色，发现CUL1在结直肠癌组织中高表达，并与患者预后不良相关，提示其可能作为肿瘤治疗的潜在靶点。
3. **文献名称**：*"Characterization of a novel CUL1 monoclonal antibody for functional studies in ubiquitination assays"*
**作者**：Tanaka K et al.
**摘要**：报道了一种高特异性CUL1单克隆抗体的开发与验证，证实其适用于免疫荧光、Co-IP等实验，为研究CUL1在泛素化通路中的功能提供了可靠工具。
(注：以上文献信息为示例性质，实际引用请以真实论文为准。)
CUL1 (Cullin 1) is a core component of the Skp1-Cullin 1-F-box protein (SCF) ubiquitin ligase complex, which plays a critical role in the ubiquitin-proteasome system by targeting specific proteins for degradation. As a scaffold protein, CUL1 facilitates the assembly of the SCF complex, linking F-box proteins (substrate receptors) to the RING-finger protein RBX1. which recruits E2 ubiquitin-conjugating enzymes. This process regulates diverse cellular processes, including cell cycle progression, signal transduction, and DNA repair, by controlling the turnover of key regulators like cyclins, p27. and β-catenin.
CUL1 antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunoprecipitation (IP), and immunofluorescence (IF) to investigate CUL1's involvement in diseases like cancer, neurodegenerative disorders, and developmental anomalies. For example, dysregulation of CUL1 has been implicated in tumorigenesis due to its role in degrading tumor suppressors or oncoproteins. Antibodies targeting specific epitopes (e.g., N-terminal or C-terminal regions) help distinguish post-translational modifications, such as neddylation, which is crucial for SCF complex activation.
Researchers validate CUL1 antibodies using knockout cell lines or siRNA knockdown to ensure specificity. Commercial antibodies are often raised against recombinant protein fragments or synthetic peptides. Reliable CUL1 detection aids in elucidating mechanisms of ubiquitination-dependent pathways and evaluating therapeutic strategies targeting SCF ligases.
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