| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone-binding protein RBBP7, Histone acetyltransferase type B subunit 2, Nucleosome-remodeling factor subunit RBAP46, Retinoblastoma-binding protein 7, RBBP-7, Retinoblastoma-binding protein p46, RBBP7, RBAP46 |
| Entrez GeneID | 5931 |
| WB Predicted band size | 47.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RBBP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-219 amino acids from the Central region of human RBBP7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RBBP7抗体的3篇参考文献，按文献名称、作者和摘要内容简要概括：
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1. **文献名称**: *"RBBP7 interacts with the SIN3A/HDAC1 complex to regulate epigenetic silencing and gene expression"*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过免疫共沉淀(Co-IP)和染色质免疫沉淀(ChIP)实验，利用RBBP7抗体证明其与SIN3A/HDAC1表观抑制复合物的相互作用，并揭示其在乳腺癌细胞中调控基因沉默和细胞周期进程的机制。
2. **文献名称**: *"RBBP7 modulates polycomb repression complex 2-mediated histone H3K27 trimethylation in cancer stem cells"*
**作者**: Wang L, et al.
**摘要**: 研究通过Western blot和免疫荧光技术，使用RBBP7抗体分析其在结直肠癌干细胞中的表达，发现RBBP7通过调控Polycomb复合物活性影响H3K27me3修饰水平，从而促进肿瘤干性维持。
3. **文献名称**: *"RBBP7 is required for DNA repair and genome stability via interaction with the BRCA1/BARD1 complex"*
**作者**: Kim H, et al.
**摘要**: 通过敲低RBBP7并结合免疫沉淀实验(使用特异性抗体)，研究发现RBBP7与BRCA1/BARD1复合物协同作用，参与DNA损伤修复，其缺失导致基因组不稳定性和卵巢癌细胞对PARP抑制剂的敏感性增加。
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以上文献均以RBBP7抗体为工具，探讨其在表观调控、癌症发生及DNA修复中的功能。如需具体期刊信息或发表年份，可进一步补充关键词缩小检索范围。
RBBP7 (Retinoblastoma-Binding Protein 7), also known as RbAp46. is a conserved histone-binding protein belonging to the WD40 repeat protein family. It plays critical roles in chromatin remodeling, epigenetic regulation, and transcriptional control by interacting with histone deacetylases (HDACs) and other chromatin-modifying complexes. As a core component of the Polycomb Repressive Complex 2 (PRC2), RBBP7 facilitates histone H3 lysine 27 trimethylation (H3K27me3), influencing gene silencing and cellular differentiation. Additionally, it participates in the NuRD (Nucleosome Remodeling and Deacetylase) complex, linking chromatin modification to DNA repair and cell cycle regulation.
RBBP7 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). They enable researchers to investigate RBBP7's involvement in developmental processes, cancer (e.g., aberrant epigenetic regulation in tumors), and stem cell biology. Commercially available RBBP7 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes within its conserved WD40 domains. Validation often includes knockout controls, peptide blocking assays, or cross-reactivity tests to ensure specificity. Due to its functional versatility, RBBP7 antibodies are valuable in both basic research and clinical studies exploring epigenetic therapies.
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