| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA-binding protein Raly, Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP core protein C-like 2, hnRNP associated with lethal yellow protein homolog, RALY, HNRPCL2, P542 |
| Entrez GeneID | 22913 |
| WB Predicted band size | 32.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RALY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the Central region of human RALY. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RALY抗体的3篇示例参考文献(注：内容为模拟示例，具体文献需通过学术数据库核实)：
1. **文献名称**: "RALY regulates p53-mediated apoptosis through mRNA stability control"
**作者**: Smith A et al.
**摘要**: 研究揭示了RALY蛋白通过结合p53 mRNA的3'UTR区域调控其稳定性，影响肿瘤细胞凋亡。使用RALY特异性抗体进行免疫沉淀，证实其在DNA损伤条件下的表达变化。
2. **文献名称**: "RALY as a novel oncogenic driver in colorectal cancer"
**作者**: Chen L et al.
**摘要**: 通过Western blot和免疫组化(使用抗RALY抗体)发现RALY在结直肠癌组织中高表达，其敲除可抑制Wnt/β-catenin信号通路，提示其作为潜在治疗靶点。
3. **文献名称**: "RNA-binding protein RALY promotes stress granule formation during oxidative stress"
**作者**: Tanaka K et al.
**摘要**: 利用RALY抗体进行共聚焦显微镜观察，证实RALY与TIA-1共定位，通过调控特定mRNA的翻译抑制参与氧化应激下的应激颗粒组装。
建议通过PubMed或Google Scholar以"RALY antibody"+"应用场景(如WB/IHC)"为关键词检索最新实证研究。
RALY antibodies are immunological tools used to study the RALY protein, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. RALY, also known as hnRNP CL, is an RNA-binding protein involved in mRNA processing, stability, and translation regulation. It plays roles in alternative splicing, viral RNA interactions, and cellular stress responses. Dysregulation of RALY has been linked to cancers, neurodegenerative diseases, and viral infections, making it a target for biomedical research.
RALY antibodies are primarily employed in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and interactions. They help researchers investigate RALY's functional mechanisms in RNA metabolism and its pathological implications. For example, studies using these antibodies have revealed RALY's overexpression in certain cancers, where it promotes tumor progression by regulating oncogenic transcripts. In neurodegenerative contexts, RALY antibodies aid in exploring its role in mRNA stability defects associated with conditions like Alzheimer's disease.
Commercial RALY antibodies are typically raised in hosts like rabbits or mice using immunogenic peptide sequences. Validation includes testing for specificity via knockout cell lines or siRNA knockdown. Challenges include cross-reactivity with homologous hnRNP proteins, requiring careful experimental controls. Recent advancements have improved antibody precision, supporting RALY's emerging potential as a diagnostic biomarker or therapeutic target in precision medicine.
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