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Rabbit Polyclonal GTF2B(N-term) Antibody

  • 中文名: GTF2B (N-term)抗体
  • 别    名: Transcription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
货号: IPDX31453
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesTranscription initiation factor IIB, General transcription factor TFIIB, S300-II, GTF2B, TF2B, TFIIB
Entrez GeneID2959
WB Predicted band size34.8kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis GTF2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-71 amino acids from the N-terminal region of human GTF2B.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于GTF2B(N-term)抗体的3篇参考文献示例(注:因文献数据库访问限制,以下为模拟内容,实际文献需通过学术数据库查询):

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1. **文献名称**: "Structural Insights into the Recruitment of TFIIB by the RNA Polymerase II Preinitiation Complex"

**作者**: Smith J, Doe R, Brown A

**摘要**: 本研究通过X射线晶体学解析了RNA聚合酶II与GTF2B(TFIIB)N端结构域的相互作用机制。作者利用针对GTF2B N端的特异性抗体进行免疫共沉淀实验,验证了该区域在转录起始复合体组装中的关键作用。

2. **文献名称**: "Functional Analysis of TFIIB Domains in Transcriptional Regulation"

**作者**: Lee S, Chen X, Zhang Y

**摘要**: 文章通过缺失突变实验比较了GTF2B不同结构域的功能差异。使用N端特异性抗体进行Western blot和染色质免疫沉淀(ChIP),发现N端区域对启动子定位和基础转录活性至关重要。

3. **文献名称**: "Antibody-Based Profiling of Transcription Factor Complexes in Human Cells"

**作者**: Johnson M, White T, Garcia-Blanco MA

**摘要**: 该研究系统评估了多种转录因子抗体的特异性,包括GTF2B的N端抗体。通过siRNA敲降和质谱分析,验证了该抗体在免疫荧光和蛋白质相互作用研究中的高特异性,为相关研究提供了工具支持。

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**注意事项**:

- 实际文献需通过PubMed、Google Scholar等平台检索,建议使用关键词如 **"GTF2B N-terminal antibody"** 或 **"TFIIB antibody epitope mapping"**。

- 部分抗体生产商(如Abcam、Thermo Fisher)的产品页面可能附相关引用文献,可参考其技术文档。

- 若研究聚焦特定技术(如ChIP-seq),可进一步限定关键词,如 **"GTF2B ChIP-seq antibody"**。

背景信息

The GTF2B (N-term) antibody targets the N-terminal region of General Transcription Factor II B (TFIIB), a critical component of the RNA polymerase II-mediated transcription machinery. TFIIB plays a central role in initiating eukaryotic transcription by bridging RNA polymerase II and promoter-bound TFIID, facilitating the assembly of the pre-initiation complex. Its N-terminal domain interacts with the TATA-binding protein (TBP) and aids in stabilizing the transcription start site. This antibody is commonly used to study TFIIB's expression, localization, and function in transcriptional regulation. Researchers employ it in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to investigate gene expression mechanisms, transcriptional dysregulation in diseases (e.g., cancer, developmental disorders), and cellular responses to stimuli. The antibody's specificity for the N-terminal region ensures detection of full-length TFIIB, distinguishing it from potential degradation products. As aberrant TFIIB activity is linked to transcriptional malfunctions, this tool contributes to understanding fundamental biological processes and disease pathogenesis. Its applications span molecular biology, cancer research, and studies of viral interactions that hijack host transcription machinery.

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