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Rabbit Polyclonal HIGD2A(N-term) Antibody

  • 中文名: HIGD2A (N-term)抗体
  • 别    名: HIG1 domain family member 2A, mitochondrial, RCF1 homolog B, RCF1b, HIGD2A
货号: IPDX31448
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHIG1 domain family member 2A, mitochondrial, RCF1 homolog B, RCF1b, HIGD2A
Entrez GeneID192286
WB Predicted band size11.5kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis HIGD2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human HIGD2A.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于HIGD2A (N-term)抗体的3篇参考文献示例(注:以下文献为虚拟示例,实际文献需通过数据库检索确认):

1. **文献名称**:*HIGD2A regulates mitochondrial respiration and hypoxic adaptation in cancer cells*

**作者**:Smith J, et al.

**摘要**:本研究利用HIGD2A N端特异性抗体,通过免疫印迹和免疫荧光技术,证实HIGD2A在低氧条件下定位于线粒体内膜,并调控复合物IV的组装,从而影响肿瘤细胞的能量代谢。

2. **文献名称**:*Characterization of HIGD2A protein interactions in mitochondrial stress response*

**作者**:Lee S, et al.

**摘要**:通过HIGD2A (N-term)抗体的免疫共沉淀实验,发现HIGD2A与COX3蛋白存在直接互作,揭示了其在氧化应激下维持线粒体膜稳定性的分子机制。

3. **文献名称**:*Development of a novel polyclonal antibody targeting the N-terminal domain of HIGD2A*

**作者**:Zhang R, et al.

**摘要**:报道了一种针对HIGD2A N端表位的多克隆抗体制备方法,验证了其在组织样本中的特异性表达,并应用于缺血性心脏病模型中HIGD2A的动态表达分析。

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**提示**:实际文献建议通过PubMed或Google Scholar以关键词“HIGD2A antibody N-terminal”检索,重点关注涉及抗体验证(如Western blot、IHC等)及功能机制的研究。

背景信息

The HIGD2A (N-term) antibody targets the N-terminal region of the hypoxia-inducible domain family member 2A (HIGD2A), a mitochondrial inner membrane protein implicated in cellular stress responses, particularly under hypoxic conditions. HIGD2A is involved in regulating mitochondrial electron transport chain (ETC) activity, apoptosis, and metabolic adaptation. It interacts with respiratory chain complexes, modulates ROS production, and contributes to cell survival during oxygen deprivation. The antibody is commonly used in research to study HIGD2A's expression, localization, and function via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Its specificity for the N-terminal epitope allows detection of full-length protein variants, aiding investigations into mitochondrial dynamics, cancer biology (e.g., tumor hypoxia), and diseases linked to metabolic or oxidative stress. Validated in multiple species, this tool supports mechanistic insights into how HIGD2A balances cell survival and death under stress.

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