关键信息
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基因名
ASCL1
- 应用
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别名
ASCL1；ASH1；BHLHA46；HASH1；Achaete-scute homolog 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50553
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表达区间
1-236aa
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氨基酸序列
MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF
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分子量
29.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
ASCL1, also known as achaete-scute complex homolog 1, is a critical transcription factor that plays a pivotal role in neuronal differentiation and the development of the nervous system. Its involvement in neurogenesis has garnered significant research interest, particularly in the context of neural stem cell biology and neurodevelopmental disorders. Recent studies have highlighted ASCL1's ability to promote the transition from progenitor cells to mature neurons, making it a valuable target for regenerative medicine and therapies aimed at neurodegenerative diseases. Additionally, ASCL1 has been implicated in tumorigenesis, particularly in neuroblastoma and other malignancies, where its expression correlates with aggressive phenotypes. Understanding the structure and function of ASCL1, especially through the study of its recombinant protein, is essential for elucidating its mechanisms of action and therapeutic potential. By producing ASCL1 as a recombinant protein, researchers can investigate its biochemical properties, interaction with other regulatory proteins, and role in cellular pathways. This research not only advances our understanding of neurodevelopment and cancer biology but also opens avenues for developing ASCL1-based strategies for enhancing neuronal regeneration and combating neurodegenerative diseases.
