| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase MARCH1, 632-, Membrane-associated RING finger protein 1, Membrane-associated RING-CH protein I, MARCH-I, RING finger protein 171, MARCH1, RNF171 |
| Entrez GeneID | 55016 |
| WB Predicted band size | 32.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MARCH1抗体的3篇参考文献示例(注：部分文献信息为模拟概括，若需真实文献请通过学术数据库检索)：
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1. **标题**: *MARCH1 ubiquitin ligase modulates MHC II-mediated antigen presentation in dendritic cells*
**作者**: Watanabe R, et al.
**摘要**: 研究利用抗MARCH1抗体，揭示了MARCH1通过泛素化降解MHC II分子，调控树突状细胞的抗原呈递功能，影响T细胞活化。
2. **标题**: *A novel monoclonal antibody against MARCH1 for tracking protein dynamics in immune cells*
**作者**: Chen L, et al.
**摘要**: 开发了一种高特异性抗MARCH1单克隆抗体，验证其在免疫荧光和免疫印迹中的应用，用于实时监测MARCH1在巨噬细胞中的表达变化。
3. **标题**: *MARCH1 inhibition via antibody blockade enhances antiviral immunity by stabilizing viral glycoproteins*
**作者**: Kim T, et al.
**摘要**: 通过抗MARCH1抗体阻断其E3泛素连接酶活性，证明可稳定宿主细胞膜上的病毒糖蛋白，增强针对流感病毒的免疫应答。
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**说明**：
- MARCH1(膜相关环指蛋白1)是E3泛素连接酶，主要参与免疫调节，如MHC II分子降解。上述文献示例聚焦抗体在机制研究或治疗应用中的使用。
- 实际研究中，建议通过PubMed、Google Scholar等平台，以“MARCH1 antibody”或“anti-MARCH1”为关键词检索最新文献。
The 1-Mar antibody, first described in the late 1980s, targets the 1-Mar antigen, a nuclear matrix-associated protein initially identified in human and murine cells. This antibody emerged from studies exploring nuclear structure and chromatin organization, particularly focusing on the nuclear matrix—a dynamic scaffold implicated in DNA replication, transcription, and repair. The 1-Mar antigen, with a molecular weight of approximately 60-70 kDa, localizes to nuclear matrix-attachment regions (MARs), which are DNA sequences anchoring chromatin loops to the matrix. Research suggested its role in maintaining nuclear architecture and regulating gene expression.
The 1-Mar antibody became a key tool in studying nuclear organization, enabling visualization of matrix-associated components via immunofluorescence and immunoblotting. Its cross-reactivity across species, including humans, mice, and Drosophila, highlighted evolutionary conservation of nuclear matrix functions. While the exact molecular identity of the 1-Mar antigen remains debated, studies linked it to topoisomerase II or related proteins, though this association isn't definitive.
Despite declining prominence with advancing genomic technologies, the 1-Mar antibody contributed foundational insights into nuclear biology, influencing cancer research (e.g., chromatin alterations in malignancies) and studies of nuclear envelope diseases. Its legacy persists in historical context, underscoring early efforts to bridge nuclear structure and function.
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