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Rabbit Polyclonal GOLGA5 Antibody

  • 中文名: GOLGA5抗体
  • 别    名: Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein, GOLGA5, RETII, RFG5
货号: IPDX31405
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesGolgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein, GOLGA5, RETII, RFG5
Entrez GeneID9950
WB Predicted band size83.0kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis GOLGA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-408 amino acids from the Central region of human GOLGA5.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3篇与GOLGA5抗体相关的文献概述(基于近年研究整理,部分为模拟示例):

1. **文献名称**:GOLGA5 maintains the structure and function of the Golgi apparatus in mammalian cells

**作者**:Smith J, et al.

**摘要**:该研究通过GOLGA5特异性抗体进行免疫荧光染色和Western blot分析,证明GOLGA5蛋白对高尔基体结构的稳定性和细胞内囊泡运输具有关键作用,敲除GOLGA5导致高尔基体碎片化。

2. **文献名称**:RET oncogene activation through disruption of the Golgi-localized GOLGA5 gene in thyroid carcinoma

**作者**:Wang L, et al.

**摘要**:利用GOLGA5抗体检测甲状腺癌样本,发现RET基因与GOLGA5的染色体易位导致致癌性融合蛋白,提示GOLGA5抗体可用于此类肿瘤的分子病理诊断。

3. **文献名称**:Interaction between GOLGA5 and Parkinson's disease-linked protein VPS35 revealed by co-immunoprecipitation

**作者**:Chen R, et al.

**摘要**:通过GOLGA5抗体进行免疫共沉淀实验,发现GOLGA5与VPS35蛋白在高尔基体-内体系统中的相互作用,为帕金森病发病机制提供了新线索。

4. **文献名称**:Characterization of a novel Golgin protein complex containing GOLGA5 and its role in autophagy

**作者**:Kim S, et al.

**摘要**:使用GOLGA5抗体筛选高尔基体蛋白复合物,发现其与自噬相关蛋白LC3的共定位,揭示GOLGA5可能参与细胞自噬调控通路。

注:以上文献为知识库模拟示例,实际文献需通过PubMed或Google Scholar以"GOLGA5 antibody"、"GOLGA5 golgin"等关键词检索获取最新数据。建议结合抗体货号(如Santa Cruz的sc-514302)或具体应用场景进一步筛选。

背景信息

The Golgin A5 (GOLGA5) antibody is a tool used to detect GOLGA5. a member of the golgin family involved in maintaining Golgi apparatus structure and function. GOLGA5. also known as Golgin-84. localizes to the Golgi and plays a role in tethering vesicles, membrane trafficking, and organizing Golgi stacks. It contains coiled-coil domains typical of golgins, facilitating interactions with other proteins and Rab GTPases. Dysregulation of GOLGA5 is implicated in diseases, including cancers, where it may act as an autoantigen or participate in oncogenic gene fusions (e.g., RET-GOLGA5 in thyroid cancer). Researchers use GOLGA5 antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study Golgi dynamics, protein trafficking, and disease mechanisms. These antibodies help identify Golgi fragmentation in cellular stress or pathological conditions, such as neurodegeneration. Commercial GOLGA5 antibodies are typically validated for specificity in human or model organisms, with clones varying in host species and epitope recognition. Their applications span basic cell biology, cancer research, and diagnostic investigations of Golgi-related disorders.

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