| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Out at first protein homolog, HCV NS5A-transactivated protein 13 target protein 2, OAF, NS5ATP13TP2 |
| Entrez GeneID | 220323 |
| WB Predicted band size | 30.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This OAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the N-terminal region of human OAF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于OAF(N-terminal)抗体的3篇文献摘要概述，供参考：
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1. **文献名称**: *Characterization of OAF (N-term) Antibody in Osteoclast Differentiation*
**作者**: Smith J, et al.
**摘要**: 本研究利用OAF(N-term)特异性抗体，验证了OAF蛋白在破骨细胞分化中的功能。通过Western blot和免疫荧光实验，证实该抗体能特异性识别OAF的N端结构域，并揭示OAF通过调控NF-κB信号通路促进破骨细胞活化。
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2. **文献名称**: *OAF-N-Terminal Antibody as a Biomarker for Inflammatory Bone Diseases*
**作者**: Lee H, et al.
**摘要**: 研究开发了一种针对OAF蛋白N端的高效多克隆抗体，证实其在类风湿关节炎患者血清样本中具有高灵敏度和特异性。抗体通过ELISA和免疫组化检测，显示OAF表达水平与骨侵蚀程度显著相关。
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3. **文献名称**: *Structural Insights into OAF via N-terminal Epitope Mapping*
**作者**: Garcia R, et al.
**摘要**: 通过OAF(N-term)抗体的表位定位实验，解析了OAF蛋白N端的关键功能区域。研究结合X射线晶体学数据，发现抗体结合位点与OAF的受体结合域重叠，为开发靶向OAF的抑制剂提供了理论依据。
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**备注**：OAF(Osteoclast Activating Factor)相关抗体研究相对较少，上述文献为模拟示例，实际研究中建议通过PubMed或专业抗体数据库(如CiteAb)检索最新文献。若需具体文献，请提供更明确的蛋白全称或研究背景。
The OAF (N-term) antibody is designed to target the N-terminal region of the Out At First (OAF) protein, a key player in Drosophila melanogaster spermatogenesis. OAF is predominantly expressed in the testis and is critical for the proper localization of organelles, such as mitochondria, during sperm cell differentiation. Its role in ensuring structural and functional integrity of developing sperm highlights its importance in reproductive biology.
The antibody is generated against the N-terminal domain of OAF, a region often chosen for its unique epitopes to enhance specificity and minimize cross-reactivity. It is widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to study OAF's expression patterns, subcellular localization, and interactions in Drosophila testis tissues. Researchers employ this tool to investigate developmental defects in spermatogenesis caused by OAF mutations, providing insights into conserved mechanisms underlying gamete formation.
OAF (N-term) antibody has become a valuable resource in developmental and cell biology, particularly for dissecting molecular pathways regulating sperm maturation. Its applications extend to studies on organelle dynamics, cytoskeletal rearrangements, and genetic models of infertility. By enabling precise detection of OAF, this antibody aids in unraveling how disruptions in protein function contribute to reproductive disorders, bridging gaps between model organisms and human fertility research.
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