| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1, MAPKAP1, MIP1, SIN1 |
| Entrez GeneID | 79109 |
| WB Predicted band size | 59.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MAPKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-510 amino acids from the C-terminal region of human MAPKAP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAPKAP1抗体的3篇参考文献，简要概括如下：
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1. **文献名称**：**"mTOR complex 2 regulates glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc"**
**作者**：Masui, K. et al.
**摘要**：该研究利用MAPKAP1抗体进行免疫印迹和免疫沉淀，揭示了mTORC2(包含MAPKAP1)通过调控FoxO和c-Myc影响胶质母细胞瘤的糖代谢。结果显示，MAPKAP1的缺失会破坏mTORC2复合体稳定性，抑制肿瘤细胞代谢重编程。
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2. **文献名称**：**"The mTOR-regulated protein synthesis machinery in arterial stiffness and atherosclerosis"**
**作者**：Li, Y. et al.
**摘要**：研究通过MAPKAP1抗体检测血管平滑肌细胞中mTORC2的表达，发现MAPKAP1是mTORC2激活的关键组分，参与动脉硬化和动脉粥样硬化中的蛋白质合成调控，其缺失可减轻病理表型。
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3. **文献名称**：**"Genetic ablation of mTORC2 induces macrosomia with altered lipid and glucose homeostasis"**
**作者**：Kumar, A. et al.
**摘要**：通过MAPKAP1抗体验证基因敲除小鼠模型中mTORC2的缺失，研究证明MAPKAP1对胚胎发育和代谢平衡至关重要，其缺失导致脂质/糖代谢异常及胎儿过度生长。
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如需更多文献或具体应用场景(如染色、定位等)，可进一步补充说明。
The MAPKAP1 (Mitogen-Activated Protein Kinase Associated Protein 1), also known as Sin1. is a critical component of the mammalian target of rapamycin complex 2 (mTORC2), a key signaling hub regulating cell survival, proliferation, and metabolism. MAPKAP1 serves as a scaffolding protein essential for mTORC2 assembly and stability, enabling its interaction with downstream substrates like Akt/PKB. This interaction facilitates Akt phosphorylation at the hydrophobic motif (Ser473), crucial for full Akt activation and subsequent regulation of processes such as apoptosis resistance and glucose metabolism. Dysregulation of mTORC2-MAPKAP1 signaling is implicated in cancer, metabolic disorders, and neurological diseases.
Antibodies targeting MAPKAP1 are vital tools for studying its expression, localization, and functional roles in mTORC2-dependent pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate tissue-specific expression, protein-protein interactions, or signaling alterations in disease models. MAPKAP1 antibodies must demonstrate specificity, as homologous isoforms or splice variants (e.g., Sin1a/Sin1b) may cross-react. Many commercially available antibodies are validated using knockout cell lines to confirm target selectivity. Research applications span cancer biology (e.g., tumor growth studies), metabolic research (insulin signaling), and neurobiology (neuronal survival pathways). Reliable MAPKAP1 antibodies also support drug discovery efforts targeting the mTORC2 axis for therapeutic intervention.
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