| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase Nek3, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, Nek3 |
| Entrez GeneID | 23954 |
| WB Predicted band size | 57.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Nek3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-474 amino acids from the C-terminal region of mouse Nek3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于 **Mouse Nek3抗体** 的模拟参考文献(信息为示例，建议通过学术数据库验证准确性)：
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1. **文献名称**：*Nek3 Kinase Regulates Sperm Flagellar Formation and Male Fertility in Mice*
**作者**：Huang, Y. et al.
**摘要**：本研究利用特异性抗Mouse Nek3抗体，通过免疫荧光和Western blot分析，揭示了Nek3在小鼠精子鞭毛形成中的关键作用。基因敲除小鼠模型显示Nek3缺失导致精子运动缺陷和不育。
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2. **文献名称**：*Nek3 Modulates Breast Cancer Cell Migration via Microtubule Dynamics*
**作者**：Shin, S.B. et al.
**摘要**：通过抗Mouse Nek3抗体进行免疫沉淀和siRNA敲低实验，发现Nek3通过调控微管稳定性影响乳腺癌细胞迁移。研究提示Nek3可能成为肿瘤转移治疗的潜在靶点。
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3. **文献名称**：*Neuronal Localization and Function of Nek3 in Mouse Brain Development*
**作者**：Smith, J.R. et al.
**摘要**：使用抗Mouse Nek3抗体的免疫组化分析显示，Nek3在小鼠海马神经元中高表达，并参与突触可塑性调控。基因沉默实验表明其缺失导致学习记忆行为异常。
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4. **文献名称**：*Nek3 Deficiency Disrupts G2/M Phase Transition in Mouse Embryonic Fibroblasts*
**作者**：Chen, L. et al.
**摘要**：通过抗Mouse Nek3抗体的Western blot和流式细胞术，研究发现Nek3缺失导致小鼠胚胎纤维母细胞G2/M期阻滞，表明其在细胞周期调控中的必要性。
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**注意**：以上文献信息为示例，实际文献需通过 **PubMed/Google Scholar** 等平台检索关键词(如“Mouse Nek3 antibody”、“Nek3 kinase function”)。若需具体文献，建议补充实验背景或抗体货号进一步筛选。
**Background on Mouse Nek3 Antibody**
The Mouse Nek3 antibody is a tool designed to detect Nek3 (NIMA-related kinase 3), a serine/threonine kinase belonging to the Nek family, which regulates cell cycle progression, cytoskeletal organization, and signal transduction. Nek3 is less characterized compared to other family members (e.g., Nek2. Nek6) but is implicated in neuronal development, cellular morphogenesis, and stress response pathways. It is predominantly expressed in the brain, testes, and lymphoid tissues.
This antibody is typically developed using immunogens derived from specific regions of the mouse Nek3 protein, ensuring specificity for mouse-derived samples. It is validated for applications like Western blotting, immunofluorescence, and immunohistochemistry. Research using this antibody has helped elucidate Nek3's role in modulating PI3K/Akt and MAPK signaling, as well as its interaction with proteins like β-catenin or tubulin, linking it to cancer progression and neurological disorders.
Nek3 knockout (KO) mouse studies highlight its importance in maintaining neuronal integrity and immune cell function. Dysregulation of Nek3 is associated with pathologies such as glioblastoma and Alzheimer’s disease. The antibody’s utility lies in its ability to track Nek3 expression/localization, aiding investigations into its kinase activity, post-translational modifications, and disease mechanisms. Validation often includes testing in Nek3 KO tissues to confirm absence of cross-reactivity.
In summary, the Mouse Nek3 antibody is essential for exploring the kinase’s biological functions and its relevance in health and disease.
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