| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Neuropeptide FF receptor 2, G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, Neuropeptide G-protein coupled receptor, NPFFR2, GPR74, NPFF2, NPGPR |
| Entrez GeneID | 10886 |
| WB Predicted band size | 60.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NPFFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3-32 amino acids from the N-terminal region of human NPFFR2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于NPFFR2 (N-term)抗体的3篇参考文献示例(注：文献信息为模拟示例，实际引用请核实原文)：
1. **文献名称**：*"Characterization of a novel NPFFR2 N-terminal antibody for neuroanatomical mapping in the rodent brain"*
**作者**：Elhabazi et al.
**摘要**：该研究开发了一种针对NPFFR2 N端表位的高特异性多克隆抗体，通过免疫组化和Western blot验证其在啮齿类动物脑组织中的表达，发现NPFFR2广泛分布于下丘脑、杏仁核等区域，提示其参与神经内分泌调节。
2. **文献名称**：*"NPFFR2 signaling in pain modulation: Insights from antibody-based receptor localization"*
**作者**：Mollereau et al.
**摘要**：利用NPFFR2 (N-term)抗体探究受体在脊髓背根神经节的表达，发现炎症疼痛模型中NPFFR2表达上调，提示其可能通过抑制痛觉信号传递参与镇痛通路。
3. **文献名称**：*"Interactions between NPFFR2 and opioid receptors revealed by co-immunoprecipitation studies"*
**作者**：Gouardères et al.
**摘要**：通过NPFFR2 N端抗体进行免疫共沉淀实验，证实NPFFR2与μ-阿片受体在细胞膜上存在物理相互作用，为二者在阿片耐受中的功能关联提供分子证据。
如需具体文献，建议在PubMed或Web of Science中检索关键词“NPFFR2 antibody N-terminal”或结合研究领域(如神经科学、GPCR)筛选。
The NPFFR2 (N-term) antibody is a specialized tool designed to target the N-terminal region of the Neuropeptide FF Receptor 2 (NPFFR2), a G protein-coupled receptor (GPCR) involved in modulating various physiological processes, including pain regulation, opioid tolerance, and energy homeostasis. NPFFR2 is activated by neuropeptide FF (NPFF) and related peptides, which play roles in neuroendocrine signaling and central nervous system functions. The N-terminal extracellular domain of NPFFR2 is critical for ligand binding and receptor activation, making this region a strategic focus for studying receptor-ligand interactions and downstream signaling pathways.
Antibodies targeting the N-terminal epitope are commonly used in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect NPFFR2 expression in tissues or cell lines. They enable researchers to investigate receptor localization, expression levels, and potential alterations under pathological conditions, such as chronic pain or metabolic disorders. Validation of these antibodies typically involves knockout controls or peptide blocking assays to confirm specificity. As NPFFR2 is expressed in both the central nervous system and peripheral tissues, these antibodies are valuable in neuroscience, endocrinology, and pharmacology research. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary by commercial source, allowing flexibility for experimental design.
×
